用胰岛素预处理的人二倍体细胞中的干扰素合成。
Interferon synthesis in human diploid cells pretreated with insulin.
作者信息
Azuma M, Suenaga T, Yoshida I, Mizuno F
出版信息
Antimicrob Agents Chemother. 1978 Apr;13(4):566-9. doi: 10.1128/AAC.13.4.566.
Abstract
Interferon production by Newcastle disease virus in human diploid (WI-38) cells was greatly reduced in the absence of serum. Pretreatment of the serum-free cells with insulin in excess of 1.2 IU/ml for 2 h before induction considerably increased the amount of interferon produced. No enhancement of interferon production was seen when the cells were exposed to insulin 2 h after induction or later. The insulin's effect persisted for at least 6 h after its removal, but not for 24 h. A possible mechanism of insulin action is discussed.
摘要
在无血清的情况下，人二倍体（WI-38）细胞中新城疫病毒产生的干扰素量大幅减少。在诱导前2小时用超过1.2 IU/ml的胰岛素对无血清细胞进行预处理，可显著增加干扰素的产生量。当细胞在诱导后2小时或更晚接触胰岛素时，未观察到干扰素产生的增强。胰岛素去除后，其作用至少持续6小时，但不持续24小时。本文讨论了胰岛素作用的一种可能机制。
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