用胰岛素预处理的人二倍体细胞中的干扰素合成。
Interferon synthesis in human diploid cells pretreated with insulin.
作者信息
Azuma M, Suenaga T, Yoshida I, Mizuno F
出版信息
Antimicrob Agents Chemother. 1978 Apr;13(4):566-9. doi: 10.1128/AAC.13.4.566.
Abstract
Interferon production by Newcastle disease virus in human diploid (WI-38) cells was greatly reduced in the absence of serum. Pretreatment of the serum-free cells with insulin in excess of 1.2 IU/ml for 2 h before induction considerably increased the amount of interferon produced. No enhancement of interferon production was seen when the cells were exposed to insulin 2 h after induction or later. The insulin's effect persisted for at least 6 h after its removal, but not for 24 h. A possible mechanism of insulin action is discussed.
摘要
在无血清的情况下，人二倍体（WI-38）细胞中新城疫病毒产生的干扰素量大幅减少。在诱导前2小时用超过1.2 IU/ml的胰岛素对无血清细胞进行预处理，可显著增加干扰素的产生量。当细胞在诱导后2小时或更晚接触胰岛素时，未观察到干扰素产生的增强。胰岛素去除后，其作用至少持续6小时，但不持续24小时。本文讨论了胰岛素作用的一种可能机制。
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1
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J Biol Chem. 1959 Oct;234:2754-8.
2
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Proc Soc Exp Biol Med. 1963 Feb;112:340-4. doi: 10.3181/00379727-112-28038.
3
Production of high-titered interferon in cultures of human diploid cells.在人二倍体细胞培养物中产生高滴度干扰素。
Antimicrob Agents Chemother. 1972 Dec;2(6):476-84. doi: 10.1128/AAC.2.6.476.
4
Production of crude human interferon with high specific activity.
Proc Soc Exp Biol Med. 1972 Jun;140(2):485-91. doi: 10.3181/00379727-140-36486.
5
Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.在无蛋白质和脂质的化学成分明确的合成培养基中长期培养哺乳动物细胞系。
Exp Cell Res. 1971 Aug;67(2):295-304. doi: 10.1016/0014-4827(71)90412-5.
6
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8
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