质子偶联转运系统的定量分析。金黄色葡萄球菌中的谷氨酸转运
Quantitative analysis of proton-linked transport systems. Glutamate transport in Staphylococcus aureus.
作者信息
Mitchell W J, Booth I R, Hamilton W A
出版信息
Biochem J. 1979 Nov 15;184(2):441-9. doi: 10.1042/bj1840441.
Abstract
- The magnitude of the protonmotive force in respiring Staphylococcus aureus was measured over the range of extracellular pH from 5.6 to 7.8. 2. The membrane potential remains constant at 150 mV, inside-negative, but the pH gradient decreases from 2.1 units, inside-alkaline, at pH 5.6 to zero at pH 7.5 and above. 3. The accumulation of glutamate in the soluble cell pool is pH-independent at a value equivalent to 100 mV. 4. The results of experiments studying co-transport of protons are consistent with a proton/glutamate stoichiometry of 2 and electrogenic transport across the pH range examined. 5. The amount of glutamate uptake is the result of a kinetic steady state between influx and efflux pathways. 6. Evidence is presented for the regulation of this kinetic steady state by the response of the initial rate of uptake to changes in the protonmotive force.
摘要
- 在细胞外pH值从5.6至7.8的范围内，对正在进行呼吸作用的金黄色葡萄球菌中的质子动力势大小进行了测量。2. 膜电位保持恒定在150 mV，内侧为负，但pH梯度从pH 5.6时的2.1个单位（内侧呈碱性）下降至pH 7.5及以上时的零。3. 谷氨酸在可溶性细胞池中积累的量与pH无关，相当于100 mV。4. 研究质子协同转运的实验结果与质子/谷氨酸化学计量比为2以及在所研究的pH范围内的电生成转运一致。5. 谷氨酸摄取量是流入和流出途径之间动力学稳态的结果。6. 有证据表明，通过摄取初始速率对质子动力势变化的响应来调节这种动力学稳态。
相似文献
1
Quantitative analysis of proton-linked transport systems. Glutamate transport in Staphylococcus aureus.质子偶联转运系统的定量分析。金黄色葡萄球菌中的谷氨酸转运
Biochem J. 1979 Nov 15;184(2):441-9. doi: 10.1042/bj1840441.
2
[Sources of convertible energy in Staphylococcus].[葡萄球菌中可转换能量的来源]
Ukr Biokhim Zh (1978). 1996 Nov-Dec;68(6):29-33.
3
Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli.呼吸状态下金黄色葡萄球菌和大肠杆菌中质子动力势的大小。
J Bacteriol. 1976 Jun;126(3):1224-31. doi: 10.1128/jb.126.3.1224-1231.1976.
4
Characteristics of energy-linked proton translocation in liposome reconstituted bovine cytochrome bc1 complex. Influence of the protonmotive force on the H+/e- stoichiometry.脂质体重构牛细胞色素bc1复合物中能量偶联质子转运的特征。质子动力对H⁺/e⁻化学计量的影响。
Eur J Biochem. 1992 Oct 1;209(1):475-81. doi: 10.1111/j.1432-1033.1992.tb17312.x.
5
Evidence for an electrogenic 3-deoxy-2-oxo-D-gluconate--proton co-transport driven by the protonmotive force in Escherichia coli K12.大肠杆菌K12中由质子动力驱动的电生性3-脱氧-2-氧代-D-葡萄糖酸-质子共转运的证据。
Biochem J. 1977 Nov 15;168(2):211-21. doi: 10.1042/bj1680211.
6
7
Artificially imposed electrical potentials drive L-glutamate uptake into synaptic vesicles of bovine cerebral cortex.人工施加的电势驱动L-谷氨酸摄取到牛大脑皮质的突触小泡中。
Biochem J. 1990 Apr 1;267(1):63-8. doi: 10.1042/bj2670063.
8
Mechanism of glutamate transport in Escherichia coli B. 2. Kinetics of glutamate transport driven by artificially imposed proton and sodium ion gradients across the cytoplasmic membrane.大肠杆菌B中谷氨酸转运机制。2. 由人工施加的跨细胞质膜的质子和钠离子梯度驱动的谷氨酸转运动力学。
Biochemistry. 1983 Apr 12;22(8):1959-65. doi: 10.1021/bi00277a034.
9
Mechanism of L-glutamate transport in membrane vesicles from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌膜泡中L-谷氨酸转运机制
J Bacteriol. 1989 Feb;171(2):1118-25. doi: 10.1128/jb.171.2.1118-1125.1989.
10
Membrane potential and gentamicin uptake in Staphylococcus aureus.金黄色葡萄球菌的膜电位与庆大霉素摄取
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693-7. doi: 10.1073/pnas.79.21.6693.
引用本文的文献
1
Use of a Fluorescence-Based Assay To Measure Escherichia coli Membrane Potential Changes in High Throughput.使用基于荧光的检测方法高通量测量大肠杆菌膜电位变化。
Antimicrob Agents Chemother. 2020 Aug 20;64(9). doi: 10.1128/AAC.00910-20.
2
Thermosensitive PBP2a requires extracellular folding factors PrsA and HtrA1 for MRSA β-lactam resistance.热敏感型 PBP2a 需要细胞外折叠因子 PrsA 和 HtrA1 来维持耐甲氧西林金黄色葡萄球菌的β-内酰胺类药物抗性。
Commun Biol. 2019 Nov 15;2:417. doi: 10.1038/s42003-019-0667-0. eCollection 2019.
3
Coupling of secondary active transport with a deltamu-H+.次级主动转运与△μ-H⁺的偶联
J Bioenerg Biomembr. 1983 Dec;15(6):307-19. doi: 10.1007/BF00751052.
4
Mechanism of amino Acid uptake by sugarcane suspension cells.甘蔗悬浮细胞氨基酸摄取的机制。
Plant Physiol. 1984 Dec;76(4):865-70. doi: 10.1104/pp.76.4.865.
5
Generalized kinetic analysis of ion-driven cotransport systems: a unified interpretation of selective ionic effects on Michaelis parameters.离子驱动共转运系统的广义动力学分析：对米氏参数选择性离子效应的统一解释
J Membr Biol. 1984;77(2):123-52. doi: 10.1007/BF01925862.
6
Proton motive force and Na+/H+ antiport in a moderate halophile.嗜盐适中菌中的质子动力和Na+/H+反向转运
J Bacteriol. 1983 Nov;156(2):537-44. doi: 10.1128/jb.156.2.537-544.1983.
7
Stoichiometry of catecholamine/proton exchange across the chromaffin-granule membrane.嗜铬粒细胞膜上儿茶酚胺/质子交换的化学计量学。
Biochem J. 1980 Oct 15;192(1):273-8. doi: 10.1042/bj1920273.
8
Quantitative analysis of proton-linked transport system. beta-Galactoside exit in Escherichia coli.质子偶联转运系统的定量分析。大肠杆菌中的β-半乳糖苷外排
Biochem J. 1980 May 15;188(2):467-73. doi: 10.1042/bj1880467.
9
Transmembrane pH gradient and membrane potential in Clostridium acetobutylicum during growth under acetogenic and solventogenic conditions.产乙酸和产溶剂条件下丙酮丁醇梭菌生长过程中的跨膜pH梯度和膜电位
Appl Environ Microbiol. 1985 Oct;50(4):1043-7. doi: 10.1128/aem.50.4.1043-1047.1985.
10
Energy recycling by lactate efflux in growing and nongrowing cells of Streptococcus cremoris.在生长和非生长状态的嗜热链球菌细胞中通过乳酸流出进行能量回收。
J Bacteriol. 1985 Apr;162(1):383-90. doi: 10.1128/jb.162.1.383-390.1985.
本文引用的文献
1
Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog.根据5,5-二甲基-2,4-恶唑烷二酮（DMO）的分布计算细胞内pH值；应用于犬的骨骼肌
J Clin Invest. 1959 May;38(5):720-9. doi: 10.1172/JCI103852.
2
Determination of intramitochondrial pH and intramitochondrial-extramitochondrial pH gradient of isolated heart mitochondria by the use of 5,5-dimethyl-2,4-oxazolidinedione. I. Changes during respiration and adenosine triphosphate-dependent transport of Ca++, Mg++, and Zn++.利用5,5-二甲基-2,4-恶唑烷二酮测定离体心脏线粒体的线粒体内pH值及线粒体内外pH梯度。I. 呼吸过程中以及Ca++、Mg++和Zn++的三磷酸腺苷依赖性转运过程中的变化。
J Biol Chem. 1968 May 10;243(9):2337-48.
3
Acid-base titration across the plasma membrane of Micrococcus denitrificans: factors affecting the effective proton conductance and the respiratory rate.反硝化微球菌质膜上的酸碱滴定：影响有效质子传导率和呼吸速率的因素。
J Bioenerg. 1970 Jun;1(1):61-72. doi: 10.1007/BF01516089.
4
The mechanism of the bacteriostatic action of tetrachlorosalicylanilide: a Membrane-active antibacterial compound.四氯水杨酰苯胺的抑菌作用机制：一种膜活性抗菌化合物。
J Gen Microbiol. 1968 Mar;50(3):441-58. doi: 10.1099/00221287-50-3-441.
5
Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus.金黄色葡萄球菌中能量与氨基酸转运偶联的机制。
Eur J Biochem. 1974 May 15;44(2):517-22. doi: 10.1111/j.1432-1033.1974.tb03510.x.
6
The role of hydrogen and potassium ions in the transport of acidic amino acids in Staphylococcus aureus.氢离子和钾离子在金黄色葡萄球菌酸性氨基酸转运中的作用
Biochim Biophys Acta. 1972 Apr 14;266(1):182-205. doi: 10.1016/0005-2736(72)90134-4.
7
Stoicheiometry of lactose-H+ symport across the plasma membrane of Escherichia coli.乳糖-H⁺同向转运体跨大肠杆菌质膜的化学计量学
Biochem J. 1973 Mar;132(3):587-92. doi: 10.1042/bj1320587.
8
A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N'-dicyclohexylcarbodimide.粪肠球菌中的跨膜pH梯度：起源以及质子导体和N,N'-二环己基碳二亚胺对其的消散
Biochim Biophys Acta. 1970;196(2):235-44. doi: 10.1016/0005-2736(70)90011-8.
9
The concentration of glycine by preparations of the yeast Saccharomyces Carlsbergensis depleted of adenosine triphosphate: Effects of proton gradients and uncoupling agents.缺乏三磷酸腺苷的卡尔斯伯酵母制剂中甘氨酸的浓度：质子梯度和解偶联剂的影响
Biochem J. 1976 Mar 15;154(3):669-76. doi: 10.1042/bj1540669.
10
Resolution of the multiplicity of the glutamate and aspartate transport systems of Escherichia coli.大肠杆菌谷氨酸和天冬氨酸转运系统多样性的解析
J Biol Chem. 1977 Dec 25;252(24):9055-64.
Zotero 插件