质子偶联转运系统的定量分析。金黄色葡萄球菌中的谷氨酸转运

Mitchell W J, Booth I R, Hamilton W A

Biochem J. 1979 Nov 15;184(2):441-9. doi: 10.1042/bj1840441.

The magnitude of the protonmotive force in respiring Staphylococcus aureus was measured over the range of extracellular pH from 5.6 to 7.8. 2. The membrane potential remains constant at 150 mV, inside-negative, but the pH gradient decreases from 2.1 units, inside-alkaline, at pH 5.6 to zero at pH 7.5 and above. 3. The accumulation of glutamate in the soluble cell pool is pH-independent at a value equivalent to 100 mV. 4. The results of experiments studying co-transport of protons are consistent with a proton/glutamate stoichiometry of 2 and electrogenic transport across the pH range examined. 5. The amount of glutamate uptake is the result of a kinetic steady state between influx and efflux pathways. 6. Evidence is presented for the regulation of this kinetic steady state by the response of the initial rate of uptake to changes in the protonmotive force.

在细胞外pH值从5.6至7.8的范围内，对正在进行呼吸作用的金黄色葡萄球菌中的质子动力势大小进行了测量。2. 膜电位保持恒定在150 mV，内侧为负，但pH梯度从pH 5.6时的2.1个单位（内侧呈碱性）下降至pH 7.5及以上时的零。3. 谷氨酸在可溶性细胞池中积累的量与pH无关，相当于100 mV。4. 研究质子协同转运的实验结果与质子/谷氨酸化学计量比为2以及在所研究的pH范围内的电生成转运一致。5. 谷氨酸摄取量是流入和流出途径之间动力学稳态的结果。6. 有证据表明，通过摄取初始速率对质子动力势变化的响应来调节这种动力学稳态。

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核心技术专利：CN118964589B侵权必究

Mitchell W J, Booth I R, Hamilton W A

Biochem J. 1979 Nov 15;184(2):441-9. doi: 10.1042/bj1840441.

The magnitude of the protonmotive force in respiring Staphylococcus aureus was measured over the range of extracellular pH from 5.6 to 7.8. 2. The membrane potential remains constant at 150 mV, inside-negative, but the pH gradient decreases from 2.1 units, inside-alkaline, at pH 5.6 to zero at pH 7.5 and above. 3. The accumulation of glutamate in the soluble cell pool is pH-independent at a value equivalent to 100 mV. 4. The results of experiments studying co-transport of protons are consistent with a proton/glutamate stoichiometry of 2 and electrogenic transport across the pH range examined. 5. The amount of glutamate uptake is the result of a kinetic steady state between influx and efflux pathways. 6. Evidence is presented for the regulation of this kinetic steady state by the response of the initial rate of uptake to changes in the protonmotive force.

在细胞外pH值从5.6至7.8的范围内，对正在进行呼吸作用的金黄色葡萄球菌中的质子动力势大小进行了测量。2. 膜电位保持恒定在150 mV，内侧为负，但pH梯度从pH 5.6时的2.1个单位（内侧呈碱性）下降至pH 7.5及以上时的零。3. 谷氨酸在可溶性细胞池中积累的量与pH无关，相当于100 mV。4. 研究质子协同转运的实验结果与质子/谷氨酸化学计量比为2以及在所研究的pH范围内的电生成转运一致。5. 谷氨酸摄取量是流入和流出途径之间动力学稳态的结果。6. 有证据表明，通过摄取初始速率对质子动力势变化的响应来调节这种动力学稳态。