鼻病毒和脊髓灰质炎病毒的生物物理学研究。I. 病毒核糖核蛋白的形态学
Biophysical studies on rhinovirus and poliovirus. I. Morphology of viral ribonucleoprotein.
作者信息
McGregor S, Mayer H D
出版信息
J Virol. 1968 Feb;2(2):149-54. doi: 10.1128/JVI.2.2.149-154.1968.
Abstract
An explanation has been sought for the high buoyant density of rhinoviruses, which are classified as acid-sensitive picornaviruses. Heat degradation of purified preparations of rhinovirus type 1B and poliovirus type LSc leads to the extrusion of ribonucleoprotein strands. Contour lengths of these strands were measured by electron microscopy, and the molecular weights of rhinovirus and poliovirus ribonucleic acid (RNA) were determined. Values of 2 x 10(6) and 4 x 10(6) daltons were obtained for the molecular weight of poliovirus and rhinovirus RNA, respectively. This additional nucleic acid in the rhinovirion probably accounts for the increased density and may be related to the acid sensitivity of the rhinovirus.
摘要
人们一直在寻找对鼻病毒高浮力密度的解释，鼻病毒被归类为对酸敏感的微小核糖核酸病毒。1B型鼻病毒和LSc型脊髓灰质炎病毒纯化制剂的热降解导致核糖核蛋白链的挤出。通过电子显微镜测量这些链的轮廓长度，并测定鼻病毒和脊髓灰质炎病毒核糖核酸（RNA）的分子量。脊髓灰质炎病毒和鼻病毒RNA的分子量分别为2×10⁶和4×10⁶道尔顿。鼻病毒颗粒中这种额外的核酸可能是密度增加的原因，并且可能与鼻病毒的酸敏感性有关。
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