Spencer K, Price C P
Clin Chim Acta. 1979 Jul 16;95(2):263-76. doi: 10.1016/0009-8981(79)90368-1.
The applicability of commercially available antisera for use in kinetic immunoturbidimetry has been studied using the protein albumin as a simple model. The sensitivity of the kinetic immunoturbidimetric approach has been found to be comparable to published data for nephelometric systems, being able to detect concentrations of protein as low as 1 mg/l. The technique has shown to be as precise as most dye binding techniques for the measurement of albumin, producing a within batch C.V. of 1.0% and a between batch C.V. of 1.5%. The technique was found to be comparable to another immunological technique (RID). The fast assay time (30--60 sec), cost and good precision makes this the method of choice for routine albumin measurement. The extreme sensitivity of the technique of kinetic immunoturbidimetry makes the technique applicable to the estimation of a wide range of proteins in blood, urine and CSF.
以蛋白质白蛋白作为简单模型，研究了市售抗血清在动力学免疫比浊法中的适用性。已发现动力学免疫比浊法的灵敏度与已发表的关于散射比浊系统的数据相当，能够检测低至1mg/l的蛋白质浓度。该技术已证明在测量白蛋白方面与大多数染料结合技术一样精确，批内变异系数为1.0%，批间变异系数为1.5%。该技术被发现与另一种免疫技术（放射免疫扩散法）相当。快速的检测时间（30 - 60秒）、成本以及良好的精密度使其成为常规白蛋白测量的首选方法。动力学免疫比浊法技术的极高灵敏度使其适用于估计血液、尿液和脑脊液中多种蛋白质的含量。
