一种用于测量鸡抗病毒抗体的C1固定方法。
A C1-fixation method for the measurement of chicken anti-viral antibody.
作者信息
Stolfi R L, Fugmann R A, Jensen J J, Sigel M M
出版信息
Immunology. 1971 Mar;20(3):299-306.
Because of the failure of chicken antibody to activate the first component (C1) of guinea-pig complement a haemolytic system for the measurement of chicken C1 was developed. Chicken antisera to influenza and Rous sarcoma viruses were then assayed by the measurement of the fixation of chicken C1. Antibody titres obtained with this C1-fixation assay correlated well with those obtained by other methods. The haemolytic system consisted of sheep erythrocytes sensitized with chicken antibody, partially purified preparations of chicken C1, and a guinea-pig serum reagent free of C1, but containing the remaining eight components of complement. Measurement of the residual chicken C1 by this method was profoundly influenced by ionic strength during both the fixation and the lytic phases, and by the order of addition of the reagents.
由于鸡抗体无法激活豚鼠补体的第一成分（C1），因此开发了一种用于测量鸡C1的溶血系统。然后通过测量鸡C1的固定情况来检测针对流感病毒和劳斯肉瘤病毒的鸡抗血清。通过这种C1固定试验获得的抗体效价与通过其他方法获得的结果相关性良好。该溶血系统由用鸡抗体致敏的绵羊红细胞、部分纯化的鸡C1制剂以及不含C1但含有其余八种补体成分的豚鼠血清试剂组成。用这种方法测量残留的鸡C1时，在固定阶段和溶解阶段，离子强度以及试剂的添加顺序都会对测量结果产生深远影响。
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