Carrick L, Berk R S
J Bacteriol. 1971 Apr;106(1):250-6. doi: 10.1128/jb.106.1.250-256.1971.
Electron microscopy of sectioned cells of Pseudomonas aeruginosa treated with a double fixative in N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid revealed a number of membranous inclusions varying in size and morphology. One round, electron transparent form was frequently observed which did not routinely appear to be attached to the cytoplasmic membrane and varied in size from 120 to 300 nm in diameter. However, in one case, several tubular structures between an inclusion and the cytoplasmic membrane was observed. On rare occasions, a large and unusual multilayered inclusion consisting of three thick and distinct layers was also encountered. In addition, two small mesosomal structures were singly observed in cells and were situated proximal to the cytoplasmic membrane. One type appeared to consist of a single thin membrane, whereas the other type consisted of a delicate, multilayered structure.
用N-2-羟乙基哌嗪-N-2-乙烷磺酸中的双重固定剂处理铜绿假单胞菌切片细胞的电子显微镜检查显示出许多大小和形态各异的膜状内含物。经常观察到一种圆形、电子透明的形式，它通常似乎不附着在细胞质膜上，直径大小在120至300纳米之间变化。然而，在一个案例中，观察到一个内含物与细胞质膜之间有几个管状结构。在极少数情况下，还遇到了一个由三层厚且明显的层组成的大型异常多层内含物。此外，在细胞中单独观察到两个小的间体结构，它们位于靠近细胞质膜的位置。一种类型似乎由单层薄膜组成，而另一种类型则由精致的多层结构组成。
