Wright P W, Law L W
Proc Natl Acad Sci U S A. 1971 May;68(5):973-6. doi: 10.1073/pnas.68.5.973.
Humoral antibodies, specific for the SV40 tumor-specific transplantation antigen, can be detected by an in vitro cytotoxic assay. (51)Cr-labeled SV40-transformed target cells are selectively killed in the presence of specific antibody and rabbit complement. Inhibition of cytotoxicity can be used to measure the antigen concentration and specific activity on different SV40-transformed cell lines. The assay described has several advantages when compared to other methods currently available to detect the specific SV40 antigen: it is simple, rapid, objective, reproducible, and quantitative.
针对SV40肿瘤特异性移植抗原的体液抗体可通过体外细胞毒性试验检测。用(51)铬标记的SV40转化靶细胞在特异性抗体和兔补体存在的情况下被选择性杀伤。细胞毒性的抑制可用于测量不同SV40转化细胞系上的抗原浓度和比活性。与目前用于检测特异性SV40抗原的其他方法相比,所述试验有几个优点:它简单、快速、客观、可重复且定量。