Sestáková M
Folia Microbiol (Praha). 1979;24(4):318-27. doi: 10.1007/BF02926651.
Candida utilis cultivated under batch conditions in a synthetic medium with a mixture of different carbon sources utilized first D-glucose and then D-galactose, D-mannose, D-xylose, L-arabinose, ethanol and acetic acid. The effect of acetic acid was primarily a function of pH and the physiological state of the inoculum. At pH 4.5, acetic acid at a concentration of 1 g/l increased the specific growth rate, reduced time of cultivation and increased yield of the yeast dry weight. The yield from acetic acid was 61%. In the presence of a higher content of acetic acid (3--6 g/l) the yield was only 18--26%. The yield calculated only from monosaccharides increased but the yield with respect to total carbon sources was lower. The specific growth rate decreased as well. The addition of ethanol also resulted in an increase of the production and yield of the yeast dry weight but the cultivation time was prolonged. The simultaneous utilization of carbon sources of the studied mixture modelling a sulphite fermentation medium with ethanol is advantageous. However, due to physiology of the yeast, it is most suitable to cultivate a strain adapted to utilizable carbon sources in a continuous way, in the presence of their limiting concentrations in the cultivation medium.
产朊假丝酵母在含有不同碳源混合物的合成培养基中进行分批培养时，首先利用D - 葡萄糖，然后利用D - 半乳糖、D - 甘露糖、D - 木糖、L - 阿拉伯糖、乙醇和乙酸。乙酸的影响主要取决于pH值和接种物的生理状态。在pH 4.5时，浓度为1 g/l的乙酸可提高比生长速率，缩短培养时间并增加酵母干重产量。乙酸的产量为61%。当乙酸含量较高（3 - 6 g/l）时，产量仅为18 - 26%。仅由单糖计算得出的产量增加，但相对于总碳源的产量较低。比生长速率也降低。添加乙醇也会导致酵母干重产量的增加，但培养时间会延长。同时利用模拟亚硫酸盐发酵培养基并含有乙醇的研究混合物中的碳源是有利的。然而，由于酵母的生理特性，最适合在培养基中存在其限制浓度的情况下，以连续方式培养适应可利用碳源的菌株。
