多瘤假病毒颗粒。II. 宿主细胞对假病毒产生的影响。
Polyoma pseudovirions. II. Influence of host cell on pseudovirus production.
作者信息
Yelton D B, Aposhian H V
出版信息
J Virol. 1973 Nov;12(5):1065-71. doi: 10.1128/JVI.12.5.1065-1071.1973.
The type of host cell influenced the relative amounts of pseudovirions and polyoma virions produced. The infection of primary mouse embryo cells resulted in the production of particles that were predominantly pseudovirions. Infection of baby mouse kidney or 3T3D cells yielded mainly infectious polyoma virus. The length of time that infection was allowed to continue also affected the amount of pseudovirions relative to polyoma virions. The longer the viral infection was allowed to proceed, the greater the quantity of pseudovirions produced. Pseudovirion production could be correlated with the fragmentation of host cell DNA to a size of approximately 3 x 10(6) daltons. The fragmentation of host cell DNA was much more extensive in primary mouse embryo cells than in the other cell types.
宿主细胞的类型影响了伪病毒粒子和多瘤病毒粒子的相对产量。原代小鼠胚胎细胞的感染导致产生的颗粒主要是伪病毒粒子。幼鼠肾细胞或3T3D细胞的感染主要产生有感染性的多瘤病毒。允许感染持续的时间长度也影响了相对于多瘤病毒粒子的伪病毒粒子数量。病毒感染持续的时间越长，产生的伪病毒粒子数量就越多。伪病毒粒子的产生可能与宿主细胞DNA断裂成大约3×10⁶道尔顿的大小有关。宿主细胞DNA的断裂在原代小鼠胚胎细胞中比在其他细胞类型中更为广泛。
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