Margulies L, Remeza V, Rudner R
J Bacteriol. 1971 Sep;107(3):610-7. doi: 10.1128/jb.107.3.610-617.1971.
In Bacillus subtilis and Escherichia coli, pulse-labeled ribonucleic acid (RNA) synthesized during step-down growth hybridized preferentially with the heavy (H) strand of methylated albumin-Kieselguhr-fractionated deoxyribonucleic acid (DNA). At high RNA inputs, the ratio of RNA hybridized with the H strand to that hybridized with the light (L) strand was 8.7 for B. subtilis and 2.0 for E. coli. At high DNA inputs, the H/L hybridization ratio increased by a factor of two. This change in the hybridization ratio was attributable to the fraction of the pulse-labeled RNA which is in stable RNA components. The hybridization peak of pulse-labeled RNA was specifically located in the late-eluting region of the absorbance profile of the H strand. This region was considered to represent the most actively transcribing H strand templates.
在枯草芽孢杆菌和大肠杆菌中，在逐步降低生长过程中脉冲标记合成的核糖核酸（RNA）优先与甲基化白蛋白-硅藻土分级分离的脱氧核糖核酸（DNA）的重（H）链杂交。在高RNA输入量时，枯草芽孢杆菌中与H链杂交的RNA与与轻（L）链杂交的RNA的比例为8.7，大肠杆菌为2.0。在高DNA输入量时，H/L杂交比例增加了一倍。杂交比例的这种变化归因于处于稳定RNA组分中的脉冲标记RNA的比例。脉冲标记RNA的杂交峰特异性地位于H链吸光度图谱的晚洗脱区域。该区域被认为代表转录最活跃的H链模板。
