Cassuto E, Stein M, Chargaff E
Proc Natl Acad Sci U S A. 1970 May;66(1):197-203. doi: 10.1073/pnas.66.1.197.
This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase of E. coli by transcription of the following DNA templates: (a) undenatured B. subtilis DNA (yielding N-RNA); (b) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding L-RNA and H-RNA, respectively). The study of the hybridization behavior of the various RNA products showed that N-RNA, though able to form hybrids with either strand, hybridized with H-DNA to twice as great an extent as with L-DNA. The transcripts of the separated L and H fractions exhibited complete specificity with respect to complexing: L-RNA hybridized only with L-DNA, H-RNA only with H-DNA.
本文描述了借助大肠杆菌的RNA聚合酶,通过转录以下DNA模板合成的RNA标本的制备方法及其一些性质:(a) 未变性的枯草芽孢杆菌DNA(产生N-RNA);(b) 通过在甲基化白蛋白上对变性DNA进行色谱分离得到的单链组分L和H(分别产生L-RNA和H-RNA)。对各种RNA产物杂交行为的研究表明,N-RNA虽然能够与任何一条链形成杂种,但与H-DNA杂交的程度是与L-DNA杂交程度的两倍。分离出的L和H组分的转录本在杂交方面表现出完全的特异性:L-RNA仅与L-DNA杂交,H-RNA仅与H-DNA杂交。