Culp L A, Grimes W J, Black P H
J Cell Biol. 1971 Sep;50(3):682-90. doi: 10.1083/jcb.50.3.682.
Two contact-inhibited "revertant" cell lines were isolated from an SV40-transformed mouse 3T3 cell line (SV-3T3) after exposure to 5-fluoro-2'-deoxyuridine. Revertant cells resembled 3T3 cells morphologically and grew to saturation densities which were similar to those of 3T3 cells; however, revertant cells readily formed both single and multinucleated giant cells in confluent cultures. SV40 virus was rescued from revertant cells by fusion with permissive monkey cells. The rescued virus transformed 3T3 cells with the same efficiency as wild type virus, and produced transformed colonies which were phenotypically similar to those produced by wild type virus. The revertant cells also resembled normal 3T3 cells in that they contained higher quantities of sialic acid than SV-3T3 cells. An inverse correlation was found between the saturation density of cells and their sialic acid content. Collagen content, however, of revertant cells was similar to that of SV-3T3 cells. The data presented suggest that the property of contact inhibition in revertant cells is related to the sialic acid content of the plasma membrane and that changes in sialic acid content of transformed cells are not directly specified by the viral genome.
在接触5-氟-2'-脱氧尿苷后，从SV40转化的小鼠3T3细胞系（SV-3T3）中分离出两个接触抑制“回复”细胞系。回复细胞在形态上类似于3T3细胞，生长至饱和密度，这与3T3细胞相似；然而，回复细胞在汇合培养物中很容易形成单核和多核巨细胞。通过与允许的猴细胞融合，从回复细胞中拯救出SV40病毒。拯救出的病毒转化3T3细胞的效率与野生型病毒相同，并产生表型与野生型病毒产生的转化集落相似的转化集落。回复细胞也类似于正常3T3细胞，因为它们比SV-3T3细胞含有更多的唾液酸。发现细胞的饱和密度与其唾液酸含量呈负相关。然而，回复细胞的胶原蛋白含量与SV-3T3细胞相似。所呈现的数据表明，回复细胞中的接触抑制特性与质膜的唾液酸含量有关，并且转化细胞中唾液酸含量的变化并非直接由病毒基因组决定。
