回复细胞系的分离与鉴定。VI. 回复体对猿猴病毒40和鼠肉瘤病毒再次转化的敏感性。
Isolation and characterization of revertant cell lines. VI. Susceptibility of revertants to retransformation by simian virus 40 and murine sarcoma virus.
作者信息
Vogel A, Pollack R
出版信息
J Virol. 1974 Dec;14(6):1404-10. doi: 10.1128/JVI.14.6.1404-1410.1974.
Abstract
The susceptibility of two classes of revertants of Simian virus 40 (SV40)-transformed 3T3 cells to retransformation by SV40 or murine sarcoma virus (MSV) was studied. Both serum-sensitive and density-sensitive revertants are not retransformable by SV40. MSV can transform both types of revertants. The MSV-transformed revertants grow to high cell densities and form colonies when suspended in semi-solid methylcellulose medium, but are unable to grow in 1% calf serum. The MSV-transformed revertants produce infectious MSV and murine leukemia virus and possess the same number of chromosomes as the untransformed revertants.
摘要
研究了猿猴病毒40（SV40）转化的3T3细胞的两类回复突变体对SV40或鼠肉瘤病毒（MSV）再次转化的敏感性。血清敏感型和密度敏感型回复突变体都不能被SV40再次转化。MSV可以转化这两种类型的回复突变体。MSV转化的回复突变体在悬浮于半固体甲基纤维素培养基中时可生长至高密度并形成集落，但在1%小牛血清中无法生长。MSV转化的回复突变体产生传染性MSV和鼠白血病病毒，并且与未转化的回复突变体具有相同数量的染色体。
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Intracellular cyclic AMP concentration responds specifically to growth regulation by serum.细胞内的环磷酸腺苷浓度对血清的生长调节有特异性反应。
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