Kisch A L, Kelley R O, Crissman H, Paxton L
J Cell Biol. 1973 Apr;57(1):38-53. doi: 10.1083/jcb.57.1.38.
Cells of a polyoma virus transformed clonal line (Cl-I) of baby hamster kidney fibroblasts (BHK-21) were grown in medium containing 2 percent dimethylsulfoxide (DMSO). Unlike the untransformed BHK-21 cells, Cl-I cells adapted to replication in the presence of DMSO, and they exhibited a rapidly reversible phenotypic reversion of a number of properties characteristic of the transformed state. Restoration of density dependent growth inhibition with accumulation of cells in the G(1) phase of the cell cycle occurred and was associated with restoration of contact dependent behavior and with reversion of histological and ultrastructural features towards those which characterize untransformed cells. Concomitantly, Cl-I cells grown in 2 percent DMSO lost the ability to form colonies in semisolid medium. The data presented suggest that DMSO alters the expression of cellular functions which were altered as a result of viral transformation and which may be involved in cell tumorigenicity.
将幼仓鼠肾成纤维细胞（BHK - 21）的多瘤病毒转化克隆系（Cl - I）的细胞，培养于含有2%二甲基亚砜（DMSO）的培养基中。与未转化的BHK - 21细胞不同，Cl - I细胞适应于在DMSO存在的情况下进行复制，并且它们表现出许多转化状态特征性特性的快速可逆表型逆转。随着细胞在细胞周期的G(1)期积累，密度依赖性生长抑制得以恢复，这与接触依赖性行为的恢复以及组织学和超微结构特征向未转化细胞特征的逆转相关。与此同时，在2% DMSO中培养的Cl - I细胞失去了在半固体培养基中形成集落的能力。所呈现的数据表明，DMSO改变了因病毒转化而改变的细胞功能的表达，这些功能可能与细胞致瘤性有关。
