Moehring J M, Stinebring W R, Merchant D J
Appl Microbiol. 1971 Jul;22(1):102-5. doi: 10.1128/am.22.1.102-105.1971.
Seven presumed diploid and 11 established cell lines were studied for their ability to produce free interferon in response to a standardized Newcastle disease virus challenge. Interferon production was evaluated in both serum-containing and serum-free medium. The ability of these cell lines to respond to the application of a standard interferon preparation by becoming resistant to virus was also examined. The diploid lines were distinctly more efficient producers of interferon than were the established lines. They also evidenced a greater requirement for serum to produce their maximum titers, but some were able to produce good titers in serum-free medium. The diploid lines were uniformly more sensitive to the application of exogenous interferon than were the established cell lines and attained greater degrees of virus resistance, but all lines tested displayed measurable sensitivity to interferon.
对7种假定的二倍体细胞系和11种已建立的细胞系进行了研究，以考察它们在受到标准化新城疫病毒攻击后产生游离干扰素的能力。在含血清和无血清培养基中均对干扰素的产生进行了评估。还检测了这些细胞系对应用标准干扰素制剂产生反应从而对病毒产生抗性的能力。二倍体细胞系作为干扰素的生产者明显比已建立的细胞系更高效。它们在产生最高滴度时对血清的需求也更大，但有些细胞系能够在无血清培养基中产生较高滴度。二倍体细胞系对外源干扰素的应用普遍比已建立的细胞系更敏感，并且获得了更高程度的病毒抗性，但所有测试的细胞系对干扰素均表现出可测量的敏感性。
