紫外线照射对猴病毒40和腺病毒12形成T抗原能力的灭活作用。
Inactivation of T antigen-forming capacites of simian virus 40 and adenovirus 12 by ultraviolet irradiation.
作者信息
Yamamoto H, Shimojo H
出版信息
J Virol. 1971 Apr;7(4):419-25. doi: 10.1128/JVI.7.4.419-425.1971.
Abstract
Methods to measure T antigen-forming capacities of simian virus 40 (SV40) and adenovirus 12 (Ad12) were investigated, and a method to measure the capacity in terms of T antigen-forming units was employed by the use of cytosine arabinoside. Plaque-forming units and T antigen-forming units of SV40, SV40 deoxyribonucleic acid, or Ad12 were inactivated by ultraviolet (UV) irradiation at the same rate, roughly following a single-hit curve. T-antigen formation by UV-irradiated SV40 and Ad12 was enhanced in cells multiply infected and in cells in a growing state. These observations showed that it was difficult or impossible to estimate the size of the gene for T antigen by UV inactivation.
摘要
研究了测量猴病毒40（SV40）和腺病毒12（Ad12）形成T抗原能力的方法，并采用了一种通过使用阿糖胞苷以T抗原形成单位来测量该能力的方法。SV40、SV40脱氧核糖核酸或Ad12的空斑形成单位和T抗原形成单位以相同速率被紫外线（UV）照射灭活，大致呈单次打击曲线。紫外线照射的SV40和Ad12在多重感染的细胞和生长状态的细胞中T抗原形成增强。这些观察结果表明，通过紫外线灭活来估计T抗原基因的大小是困难的或不可能的。
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