Wiese W H, Lewis A M, Rowe W P
J Virol. 1970 Apr;5(4):421-6. doi: 10.1128/JVI.5.4.421-426.1970.
The simian virus 40 (SV40)-yielding variants of the adenovirus type 2 (Ad.2)-SV40 hybrid (Ad.2(++)) population were studied by means of fixed-angle equilibrium density gradient centrifugation in cesium chloride. The hybrid virions of the Ad.2(++) high-efficiency yielder population banded at densities of 0.004 g/cm(3) lighter than the nonhybrid Ad.2 virions. The degree of separation of the hybrid particles was sufficient to permit greater than 100-fold relative purification by two cycles of centrifugation. Hybrid particles that produce adenovirus plaques in African green monkey kidney cells by two-hit kinetics (one-hit kinetics when assayed on lawns of nonhybrid adenovirus) were not separable from the particles that yield SV40 virus. The hybrid particle in the Ad.2(++) low-efficiency yielder population was not separable from the nonhybrid Ad.2 virions.
利用氯化铯中的固定角度平衡密度梯度离心法，对2型腺病毒（Ad.2）-猴病毒40（SV40）杂交体（Ad.2(++)）群体中产生SV40的变体进行了研究。Ad.2(++)高效产生群体的杂交病毒粒子在密度上比非杂交Ad.2病毒粒子轻0.004 g/cm³。杂交粒子的分离程度足以通过两轮离心实现大于100倍的相对纯化。通过双打击动力学在非洲绿猴肾细胞中产生腺病毒噬菌斑的杂交粒子（在非杂交腺病毒菌苔上检测时为单打击动力学）与产生SV40病毒的粒子无法分离。Ad.2(++)低效产生群体中的杂交粒子与非杂交Ad.2病毒粒子无法分离。
