Levin M J, Crumpacker C S, Lewis A M, Oxman M N, Henry P H, Rowe W P
J Virol. 1971 Mar;7(3):343-51. doi: 10.1128/JVI.7.3.343-351.1971.
A nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), has been plaque-isolated from an Ad2-SV40 hybrid population. This virus, unlike the defective Ad-SV40 hybrid populations previously described, replicates without the aid of nonhybrid adenovirus helper. Consequently, the hybrid virus deoxyribonucleic acid (DNA) can be obtained free of nonhybrid adenovirus DNA. The DNA of the Ad2(+)ND(1) virus was shown by ribonucleic acid (RNA)-DNA hybridization to consist of nucleotide sequences complementary to Ad2- and SV40-specific RNA. Techniques of equilibrium density and rate zonal centrifugation were employed to demonstrate that these Ad2 and SV40 nucleotide sequences were linked together in the same DNA molecules by alkali-resistant bonds. Calibration curves were established relating the amount of tritium-labeled SV40-specific RNA (prepared in vitro or in vivo) bound to given amounts of SV40 DNA in a hybridization reaction, and these curves were employed to determine the equivalent amount of SV40 DNA in the Ad2(+)ND(1) molecule. From the results obtained, it was estimated that 1% of the Ad2(+)ND(1) DNA consists of SV40 nucleotide sequences.
一种无缺陷的腺病毒2（Ad2）-猴病毒40（SV40）杂交病毒，Ad2(+)ND(1)，已从Ad2-SV40杂交群体中通过噬斑分离得到。与先前描述的有缺陷的Ad-SV40杂交群体不同，这种病毒在没有非杂交腺病毒辅助的情况下就能复制。因此，可以获得不含非杂交腺病毒DNA的杂交病毒脱氧核糖核酸（DNA）。通过核糖核酸（RNA）-DNA杂交显示，Ad2(+)ND(1)病毒的DNA由与Ad2和SV40特异性RNA互补的核苷酸序列组成。采用平衡密度和速率区带离心技术来证明这些Ad2和SV40核苷酸序列通过耐碱键连接在同一DNA分子中。建立了校准曲线，将杂交反应中与给定数量的SV40 DNA结合的氚标记SV40特异性RNA（体外或体内制备）的量关联起来，并且这些曲线被用于确定Ad2(+)ND(1)分子中SV40 DNA的等效量。根据所得结果估计，Ad2(+)ND(1) DNA的1%由SV40核苷酸序列组成。
