Leak L V
J Cell Biol. 1971 Aug;50(2):300-23. doi: 10.1083/jcb.50.2.300.
The passageway for interstitial fluids and large molecules across the connective tissue lymph interface has been investigated in dermal lymphatic capillaries in the ears of guinea pigs. Numerous endothelial cells overlap extensively at their margins and lack adhesion devices at many points. The observations suggest that these sites are free to move as a result of slight pressure changes. Immediately following interstitial injections of tracer particles (ferritin, thorium, carbon, and latex spheres), many of the overlapped endothelial cells are separated and thus passageways are provided between the interstitium and lymphatic lumen. Tracer particles also occur in plasmalemmal invaginations along both connective tissue and luminal fronts. All of the tracer particles accumulate within large autophagic-like vacuoles. Very few particles of ferritin are observed in the endothelium after 24 hr; however, the vesicles containing the nonprotein tracer particles (carbon, thorium, and latex) increase in size and content and remain within the lymphatic endothelial cells up to 6 months. The role of vesicles in the transport of large molecules and particles is discussed in relation to the accretion of tracer particles within large vesicles and autophagic-like vacuoles in the endothelial cytoplasm.
在豚鼠耳部的真皮淋巴管中，研究了间质液和大分子穿过结缔组织-淋巴界面的通道。许多内皮细胞在其边缘广泛重叠，并且在许多点缺乏黏附装置。这些观察结果表明，由于轻微的压力变化，这些部位可以自由移动。在间质注射示踪颗粒（铁蛋白、钍、碳和乳胶球）后，许多重叠的内皮细胞会分离，从而在间质和淋巴管腔之间形成通道。示踪颗粒也出现在沿结缔组织和管腔前沿的质膜内陷处。所有示踪颗粒都积聚在大型自噬样液泡中。24小时后，在内皮细胞中观察到的铁蛋白颗粒非常少；然而，含有非蛋白质示踪颗粒（碳、钍和乳胶）的囊泡大小和内容物增加，并在淋巴内皮细胞内保留长达6个月。结合示踪颗粒在内皮细胞质中的大型囊泡和自噬样液泡内的积聚情况，讨论了囊泡在大分子和颗粒运输中的作用。
