Kieff E D, Bachenheimer S L, Roizman B
J Virol. 1971 Aug;8(2):125-32. doi: 10.1128/JVI.8.2.125-132.1971.
Studies of the size, composition, and structure of the deoxyribonucleic acid (DNA) of the F and G prototypes of herpes simplex virus (HSV) subtypes 1 and 2 (HSV-1 and HSV-2) showed the following. (i) As previously reported by Good-heart et al. HSV-1 and HSV-2 DNA have a buoyant density of 1.726 and 1.728 g/cm(3), corresponding to 67 and 69 guanine +/- cytosine moles per cent, respectively. The difference in guanine plus cytosine content of the DNA species was confirmed by the finding of a 1 C difference in T(m). (ii) The DNA from purified virus on cocentrifugation with T4 DNA in neutral sucrose density gradients sedimented at 55S, corresponding to 99 +/- 5 million daltons in molecular weight. HSV-1 and HSV-2 DNA could not be differentiated with respect to size. (iii) Cosedimentation of alkali-denatured DNA from purified virus with T4 DNA on alkaline sucrose density gradients consistently yielded several bands of single-stranded HSV DNA ranging from fragments 7 x 10(6) daltons to intact strands 48 x 10(6) daltons in molecular weight.
对1型和2型单纯疱疹病毒（HSV - 1和HSV - 2）F和G原型的脱氧核糖核酸（DNA）的大小、组成和结构研究结果如下：（i）如古德哈特等人之前所报道，HSV - 1和HSV - 2 DNA的浮力密度分别为1.726和1.728 g/cm³，分别对应67%和69%的鸟嘌呤±胞嘧啶摩尔百分比。DNA种类中鸟嘌呤加胞嘧啶含量的差异通过熔解温度（Tm）相差1℃得以证实。（ii）纯化病毒的DNA与T4 DNA在中性蔗糖密度梯度中共离心时，沉降系数为55S，分子量对应99±5百万道尔顿。HSV - 1和HSV - 2 DNA在大小方面无法区分。（iii）纯化病毒的碱变性DNA与T4 DNA在碱性蔗糖密度梯度上共沉降，始终产生几条单链HSV DNA条带，分子量范围从7×10⁶道尔顿的片段到48×10⁶道尔顿的完整链。
