单纯疱疹病毒：通过复性动力学研究基因组大小和冗余度。
Herpes vimplex virus: genome size and redundancy studied by renaturation kinetics.
作者信息
Frenkel N, Roizman B
出版信息
J Virol. 1971 Oct;8(4):591-3. doi: 10.1128/JVI.8.4.591-593.1971.
Abstract
Herpes simplex virus subtype 1 deoxyribonucleic acid (DNA) was sheared in a French press to uniform fragments, denatured by heating, then allowed to reassociate. The renaturation reaction followed second-order kinetics with a single rate constant indicating that at least 95% of the genome was unique and that repetitive sequences, if present, were not detectable by this technique. The kinetic complexity of the herpes simplex genome was determined by DNA renaturation kinetics to be (95 +/- 1) x 10(6) daltons. Since this value is in excellent agreement with the molecular weight of viral DNA [(99 +/- 5) x 10(6) daltons] obtained from velocity sedimentation studies, it is concluded that virions contain only one species of double-stranded DNA molecules 95 x 10(6) to 99 x 10(6) daltons in molecular weight.
摘要
单纯疱疹病毒1型脱氧核糖核酸（DNA）在法国压榨机中被剪切成均匀的片段，通过加热使其变性，然后让其重新结合。复性反应遵循二级动力学，具有单一速率常数，这表明至少95%的基因组是独特的，并且如果存在重复序列，用该技术无法检测到。通过DNA复性动力学确定单纯疱疹基因组的动力学复杂度为（95±1）×10⁶道尔顿。由于该值与通过速度沉降研究获得的病毒DNA分子量[（99±5）×10⁶道尔顿]非常一致，因此得出结论，病毒粒子仅包含一种分子量为95×10⁶至99×10⁶道尔顿的双链DNA分子。
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