对在连续培养中以甘油、铁、铵、镁或磷酸盐作为生长限制营养物培养的产朊假丝酵母的线粒体进行比较。

Mitochondria prepared from Torulopsis utilis grown in a chemostat with iron-limited growth were found to lack energy conservation but not electron flow in that segment of the respiratory chain leading from intramitochondrial NADH to the cytochromes [i.e. the site 1 segment (Lehninger, 1964)]. 2. Site 1 energy conservation was present in mitochondria prepared from cells grown under conditions of limitation by glycerol, ammonium and magnesium. Phosphate-limited growth resulted in mitochondrial preparations without respiratory control. 3. Mitochondria from cells grown under conditions of iron limitation were insensitive to the respiratory inhibitor piericidin A, whereas sensitivity was present in mitochondria prepared from glycerol-, ammonium-, magnesium- or phosphate-limited cells. 4. These observations are considered to provide indirect evidence for a role of non-haem iron in the mechanism of energy conservation and also piericidin A sensitivity in T. utilis mitochondria. 5. A readily constructed and inexpensive pH-measuring and -controlling circuit is described for use with continuous-culture apparatus.

发现从在恒化器中培养且铁限制生长的产朊假丝酵母制备的线粒体，在从线粒体内NADH到细胞色素的呼吸链片段中（即1号位点片段，莱宁格，1964年）缺乏能量守恒但电子流正常。2. 从在甘油、铵和镁限制条件下生长的细胞制备的线粒体中存在1号位点能量守恒。磷酸盐限制生长导致线粒体提取物没有呼吸控制。3. 铁限制条件下生长的细胞的线粒体对呼吸抑制剂杀稻瘟菌素A不敏感，而从甘油、铵、镁或磷酸盐限制的细胞制备的线粒体中存在敏感性。4. 这些观察结果被认为提供了间接证据，证明非血红素铁在产朊假丝酵母线粒体能量守恒机制以及杀稻瘟菌素A敏感性中发挥作用。5. 描述了一种易于构建且廉价的pH测量和控制电路，用于连续培养设备。

A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient.