西霉素和硫链丝菌素对氨酰tRNA以及因子G与核糖体结合的抑制作用。
Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes.
作者信息
Modolell J, Cabrer B, Parmeggiani A, Vazquez D
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1796-800. doi: 10.1073/pnas.68.8.1796.
Abstract
Siomycin, a peptide antibiotic that interacts with the 50S ribosomal subunit and inhibits binding of factor G, is shown also to inhibit binding of aminoacyl-tRNA; however, it does not impair binding of fMet-tRNA and completion of the initiation complex. Moreover, unlike other inhibitors of aminoacyl-tRNA binding (tetracycline, sparsomycin, and streptogramin A), siomycin completely abolishes the GTPase activity associated with the binding of aminoacyl-tRNA catalyzed by factor T(u). A single-site interaction of siomycin appears to be responsible for its effect on both the binding of the aminoacyl-tRNA-T(u)-GTP complex and that of factor G.
摘要
西霉素是一种与50S核糖体亚基相互作用并抑制因子G结合的肽抗生素，它还被证明能抑制氨酰基-tRNA的结合；然而，它并不损害甲酰甲硫氨酰-tRNA的结合以及起始复合物的形成。此外，与其他氨酰基-tRNA结合抑制剂（四环素、稀疏霉素和链阳性菌素A）不同，西霉素完全消除了与因子T(u)催化的氨酰基-tRNA结合相关的GTPase活性。西霉素的单点相互作用似乎是其对氨酰基-tRNA-T(u)-GTP复合物和因子G结合产生影响的原因。
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本文引用的文献
1
CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS.大肠杆菌提取物中一种核糖体连接的鸟苷三磷酸酶的特性分析
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1462-9. doi: 10.1073/pnas.52.6.1462.
2
RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
3
Substrate and antibiotic binding sites at the peptidyl transferase centre of E. coli ribosomes: Binding of UACCA-Leu to 50 S subunits.大肠杆菌核糖体肽基转移酶中心的底物和抗生素结合位点：UACCA-亮氨酸与50 S亚基的结合
FEBS Lett. 1971 Mar 16;13(4):247-251. doi: 10.1016/0014-5793(71)80546-x.
4
Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex.硫链丝菌素对核糖体结合鸟嘌呤核苷酸复合物形成的抑制作用。
FEBS Lett. 1970 Dec;11(3):149-152. doi: 10.1016/0014-5793(70)80515-4.
5
Stoichiometry of aminoacyl-transfer RNA binding and GTP cleavage during chain elongation and translocation.链延伸和转位过程中氨酰基转移RNA结合与GTP裂解的化学计量学。
Nature. 1969 Aug 16;223(5207):697-701. doi: 10.1038/223697a0.
6
Bacteriophage f2 RNA: control of translation and gene order.噬菌体f2 RNA：翻译控制与基因顺序
Nature. 1968 Oct 26;220(5165):345-50. doi: 10.1038/220345a0.
7
Studies on translocation. VI. Thiostrepton prevents the formation of a ribosome-G factor-guanine nucleotide complex.易位研究。VI. 硫链丝菌素可阻止核糖体 - G因子 - 鸟嘌呤核苷酸复合物的形成。
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1406-11. doi: 10.1016/0006-291x(70)90543-7.
8
Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.多肽链起始：噬菌体R17 RNA中三个核糖体结合位点的核苷酸序列
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9
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Nature. 1966 Jul 23;211(5047):380-4. doi: 10.1038/211380a0.
10
Studies on the peptidyl tRNA translocase from rat liver.对大鼠肝脏肽基转移核糖核酸转位酶的研究。
Cold Spring Harb Symp Quant Biol. 1969;34:347-56. doi: 10.1101/sqb.1969.034.01.041.
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