延伸因子EF Tu和EF G在核糖体上的相关位点相互作用。
Elongation factors EF Tu and EF G interact at related sites on ribosomes.
作者信息
Miller D L
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):752-5. doi: 10.1073/pnas.69.3.752.
Abstract
The binding of elongation factor EF G to ribosomes inhibits the subsequent reaction of the ribosomes with the ternary complex aminoacyl-tRNA.EF Tu.GTP. Both the hydrolysis of GTP and the binding of aminoacyl-tRNA to ribosomes are nearly abolished by the previous binding of factor EF G to ribosomes in the presence of either fusidic acid plus either GTP or a nonhydrolyzable analog of GTP. The results suggest that each elongation factor binds to the same region on the ribosome. The GTPase activities of both factors EF G and EF Tu may be activated by interaction at the same ribosomal site, as has been previously suggested by others.
摘要
延伸因子EF G与核糖体的结合会抑制核糖体随后与三元复合物氨酰 - tRNA.EF Tu.GTP的反应。在存在夫西地酸加上GTP或GTP的非水解类似物的情况下，因子EF G先前与核糖体的结合几乎完全消除了GTP的水解以及氨酰 - tRNA与核糖体的结合。结果表明，每个延伸因子都结合到核糖体上的同一区域。正如其他人之前所提出的，因子EF G和EF Tu的GTP酶活性可能通过在同一核糖体位点的相互作用而被激活。
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本文引用的文献
1
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J Biol Chem. 1970 Nov 10;245(21):5662-7.
2
Translocation of messenger RNA and "accommodation" of fMet-tRNA.信使核糖核酸的易位及甲酰甲硫氨酰转运核糖核酸的“容纳”
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1791-5. doi: 10.1073/pnas.68.8.1791.
3
Movement of the ribosome along the messenger ribonucleic acid during protein synthesis.蛋白质合成过程中核糖体沿信使核糖核酸的移动。
Biochemistry. 1971 Nov 23;10(24):4410-21. doi: 10.1021/bi00800a010.
4
Protection of ribosomes from thiostrepton inactivation by the binding of G factor and guanosine diphosphate.通过G因子和二磷酸鸟苷的结合保护核糖体免受硫链丝菌素失活。
Biochemistry. 1971 Nov 23;10(24):4404-9. doi: 10.1021/bi00800a009.
5
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7
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