延伸因子EF G对氨酰tRNA与核糖体结合的抑制作用。
Inhibition by elongation factor EF G of aminoacyl-tRNA binding to ribosomes.
作者信息
Cabrer B, Vázquez D, Modolell J
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):733-6. doi: 10.1073/pnas.69.3.733.
Abstract
Elongation factor G (EF G), bound to ribosomes either with GMPPCP or with fusidic acid and GDP, inhibits elongation factor Tu (EF Tu)-dependent binding of Phe-tRNA on the ribosome-poly(U) complex and binding of Ala-tRNA on the initiation complex formed with RNA from bacteriophage R17; GTP hydrolysis associated with Phe-tRNA binding is also inhibited. Moreover, nonenzymic binding of Phe-tRNA at high Mg(++) concentration is completely blocked by EF G. Thus, EF G appears to bind at a site that overlaps or interacts with the ribosomal A-site.
摘要
延伸因子G（EF G），与结合了鸟苷5'-（β，γ-亚甲基）三磷酸（GMPPCP）或结合了夫西地酸和鸟苷二磷酸（GDP）的核糖体结合时，会抑制延伸因子Tu（EF Tu）依赖的苯丙氨酰 - tRNA在核糖体 - 聚尿苷酸（poly(U)）复合物上的结合，以及丙氨酰 - tRNA在与噬菌体R17的RNA形成的起始复合物上的结合；与苯丙氨酰 - tRNA结合相关的鸟苷三磷酸（GTP）水解也受到抑制。此外，在高镁离子（Mg(++)）浓度下苯丙氨酰 - tRNA的非酶促结合被EF G完全阻断。因此，EF G似乎结合在与核糖体A位点重叠或相互作用的位点上。
相似文献
1
Inhibition by elongation factor EF G of aminoacyl-tRNA binding to ribosomes.延伸因子EF G对氨酰tRNA与核糖体结合的抑制作用。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):733-6. doi: 10.1073/pnas.69.3.733.
2
Elongation factors EF Tu and EF G interact at related sites on ribosomes.延伸因子EF Tu和EF G在核糖体上的相关位点相互作用。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):752-5. doi: 10.1073/pnas.69.3.752.
3
Kirromycin, an inhibitor of protein biosynthesis that acts on elongation factor Tu.奇霉素，一种作用于延伸因子Tu的蛋白质生物合成抑制剂。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4910-4. doi: 10.1073/pnas.71.12.4910.
4
Ribosomes cannot interact simultaneously with elongation factors EF Tu and EF G.核糖体不能同时与延伸因子EF Tu和EF G相互作用。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):686-9. doi: 10.1073/pnas.69.3.686.
5
The interaction of fusidic acid with peptidyl-transfer-ribonucleic-acid - ribosome complexes.夫西地酸与肽基转移核糖核酸-核糖体复合物的相互作用。
Eur J Biochem. 1975 Sep 15;57(2):431-40. doi: 10.1111/j.1432-1033.1975.tb02318.x.
6
Binding of aminoacyl-tRNA to ribosomes promoted by elongation factor Tu. Studies on the role of GTP hydrolysis.延伸因子Tu促进氨酰tRNA与核糖体的结合。关于GTP水解作用的研究。
J Biochem. 1975 Apr;77(4):719-28. doi: 10.1093/oxfordjournals.jbchem.a130775.
7
The interaction of elongation factor G with N-acetylphenylalanyl transfer RNA-ribosome complexes.延伸因子G与N-乙酰苯丙氨酰转运RNA-核糖体复合物的相互作用。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3561-5. doi: 10.1073/pnas.70.12.3561.
8
Polypeptide-chain elongation promoted by guanyl-5'-yl imidodiphosphate.由鸟苷-5'-亚氨基二磷酸促进的多肽链延伸。
Eur J Biochem. 1976 Aug 1;67(1):257-65. doi: 10.1111/j.1432-1033.1976.tb10657.x.
9
Enzymatic binding of aminoacyl transfer ribonucleic acid to ribosomes: the study of binding sites of 2' and 3' isomers of aminoacyl transfer ribonucleic acid.氨酰基转移核糖核酸与核糖体的酶促结合：氨酰基转移核糖核酸2'和3'异构体结合位点的研究
Biochemistry. 1976 Jun 29;15(13):2759-65. doi: 10.1021/bi00658a008.
10
GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs.异聚体mRNA翻译过程中延伸因子Tu的GTP消耗
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1945-9. doi: 10.1073/pnas.92.6.1945.
引用本文的文献
1
A Possible Role of the Full-Length Nascent Protein in Post-Translational Ribosome Recycling.全长新生蛋白在翻译后核糖体循环中的可能作用。
PLoS One. 2017 Jan 18;12(1):e0170333. doi: 10.1371/journal.pone.0170333. eCollection 2017.
2
Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors.嘌呤霉素与大肠杆菌核糖体的结合。嘌呤霉素类似物和肽键形成抑制剂的影响。
Mol Biol Rep. 1973 Jun;1(1):27-32. doi: 10.1007/BF00357402.
3
4
Involvement of the N terminus of ribosomal protein L11 in regulation of the RelA protein of Escherichia coli.核糖体蛋白L11的N端参与大肠杆菌RelA蛋白的调控。
J Bacteriol. 2001 Nov;183(22):6532-7. doi: 10.1128/JB.183.22.6532-6537.2001.
5
Ribosomal function and its inhibition by antibiotics in prokaryotes.原核生物中的核糖体功能及其被抗生素抑制的情况。
Naturwissenschaften. 1980 May;67(5):234-50. doi: 10.1007/BF01054532.
6
The enzymatic binding of aminoacyl-tRNA to reticulocyte ribosomes: the stimulatory effect of donor site bound peptidyl-tRNA.氨酰基转移核糖核酸与网织红细胞核糖体的酶促结合：供体位点结合的肽基转移核糖核酸的刺激作用。
Mol Biol Rep. 1974 Dec;1(8):493-7. doi: 10.1007/BF00360677.
7
The mechanism of peptide chain termination.肽链终止的机制。
Mol Cell Biochem. 1974 Dec 20;5(3):115-26. doi: 10.1007/BF01731375.
8
Thiostrepton inhibition of initiation factor 1 activity in polypeptide chain initiation in Escherichia coli.硫链丝菌素对大肠杆菌多肽链起始中起始因子1活性的抑制作用。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4986-90. doi: 10.1073/pnas.71.12.4986.
9
Function of individual 30S subunit proteins of Escherichia coli. Effect of specific immunoglobulin fragments (Fab) on activities of ribosomal decoding sites.大肠杆菌30S亚基单个蛋白质的功能。特异性免疫球蛋白片段（Fab）对核糖体解码位点活性的影响。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):248-52. doi: 10.1073/pnas.71.2.248.
10
The requirement for ribosomal proteins L7 and L12 in peptide-chain termination.肽链终止过程中对核糖体蛋白L7和L12的需求。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):89-92. doi: 10.1073/pnas.71.1.89.
本文引用的文献
1
RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
2
Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex.硫链丝菌素对核糖体结合鸟嘌呤核苷酸复合物形成的抑制作用。
FEBS Lett. 1970 Dec;11(3):149-152. doi: 10.1016/0014-5793(70)80515-4.
3
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
4
Bacteriophage f2 RNA: control of translation and gene order.噬菌体f2 RNA：翻译控制与基因顺序
Nature. 1968 Oct 26;220(5165):345-50. doi: 10.1038/220345a0.
5
Studies on translocation. VI. Thiostrepton prevents the formation of a ribosome-G factor-guanine nucleotide complex.易位研究。VI. 硫链丝菌素可阻止核糖体 - G因子 - 鸟嘌呤核苷酸复合物的形成。
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1406-11. doi: 10.1016/0006-291x(70)90543-7.
6
Studies on translocation. IV. The hydrolysis of a single round of guanosine triphosphate in the presence of fusidic acid.转位研究。IV. 在夫西地酸存在下鸟苷三磷酸单轮水解反应
J Biol Chem. 1970 Nov 10;245(21):5662-7.
7
Specificity of mammalian transferase II binding to ribosomes.哺乳动物转移酶II与核糖体结合的特异性。
Nat New Biol. 1971 Oct 27;233(43):257-8. doi: 10.1038/newbio233257a0.
8
Studies on the peptidyl tRNA translocase from rat liver.对大鼠肝脏肽基转移核糖核酸转位酶的研究。
Cold Spring Harb Symp Quant Biol. 1969;34:347-56. doi: 10.1101/sqb.1969.034.01.041.
9
Thiostrepton, an inhibitor of 5OS ribosome subunit function.硫链丝菌素，一种50S核糖体亚基功能抑制剂。
J Bacteriol. 1970 Mar;101(3):1073-5. doi: 10.1128/jb.101.3.1073-1075.1970.
10
Protection of ribosomes from thiostrepton inactivation by the binding of G factor and guanosine diphosphate.通过G因子和二磷酸鸟苷的结合保护核糖体免受硫链丝菌素失活。
Biochemistry. 1971 Nov 23;10(24):4404-9. doi: 10.1021/bi00800a009.
Zotero 插件