正常和再生大鼠肝脏细胞核及细胞质细胞组分中的组蛋白磷酸激酶活性。
Histone phosphokinase activity in nuclear and cytoplasmic cell fractions from normal and regenerating rat livers.
作者信息
Siebert G, Ord M G, Stocken L A
出版信息
Biochem J. 1971 May;122(5):721-5. doi: 10.1042/bj1220721.
Abstract
- Liver cell fractions were prepared by non-aqueous procedures and nuclei were also obtained in a hyperosmotic sucrose medium. Histone phosphokinase activity, assayed with histone F1 as substrate, was present in the soluble fraction of the cytoplasm and also bound on to the chromatin fraction of the nucleus. 2. The activity of the enzyme increased sixfold in nuclei from regenerating livers 22h after partial hepatectomy. 3. The enzyme bound in the nucleus was only marginally activated by 1mum-3':5'-cyclic AMP which stimulated the cytoplasmic soluble enzyme fourfold. 4. Nuclei prepared by the non-aqueous technique were also able to phosphorylate histones F2a and F3 and showed histone phosphatase activity with histone F1 phosphate as substrate.
摘要
- 通过非水法制备肝细胞组分，并在高渗蔗糖培养基中获得细胞核。以组蛋白F1为底物测定的组蛋白磷酸激酶活性存在于细胞质的可溶性组分中，也结合在细胞核的染色质组分上。2. 部分肝切除术后22小时，再生肝脏细胞核中该酶的活性增加了六倍。3. 细胞核中结合的酶仅被1μM 3':5'-环磷酸腺苷轻微激活，而该物质可使细胞质可溶性酶的活性增加四倍。4. 通过非水技术制备的细胞核也能够使组蛋白F2a和F3磷酸化，并以磷酸化组蛋白F1为底物表现出组蛋白磷酸酶活性。
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本文引用的文献
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EVIDENCE FOR TWO DNA-DEPENDENT RNA POLYMERASE ACTIVITIES IN ISOLATED RAT-LIVER NUCLEI.大鼠肝脏细胞核中两种依赖DNA的RNA聚合酶活性的证据。
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Isolation of pure and unaltered liver nuclei morphology and biochemical composition.分离出形态和生化组成纯净且未改变的肝细胞核。
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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
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Studies on histones. 7. Preparative methods for histone fractions from calf thymus.组蛋白研究。7. 从小牛胸腺中制备组蛋白组分的方法。
Biochem J. 1964 Jul;92(1):55-9. doi: 10.1042/bj0920055.
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