感染不同病毒后不同时间原有细胞抗原结合基团的增加。

Increase in preexisting cellular antigen-combining groups at different times after infection with different viruses.

作者信息

Tarro G, Sabin A B

出版信息

Proc Natl Acad Sci U S A. 1970 Oct;67(2):731-7. doi: 10.1073/pnas.67.2.731.

DOI:10.1073/pnas.67.2.731

PMID:4331719

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283266/

Abstract

During the course of experiments designed to determine whether infection of normal cells with certain strains of Herpesvirus hominis (HSV) gives rise to a specific neoantigen, presumably characteristic for human malignant cells ("HeLa G"), it was found that the seeming appearance of such a new antigen actually represents only an increase in concentration of previously existing normal antigen groups. The complement fixation tests for this antigen were carried out with antibody prepared by McKenna against a fraction, purified with the fluorocarbon genetron, from HeLa cells. This antibody reacted with extracts of tissue culture cells derived from various human malignant tumors and from the spontaneously transformed WISH cell line, originally derived from normal human amnion, but not with cells derived from early-passage cultures of normal human kidney, or from primary cultures of young rabbit kidney or new-born guinea pig kidney. Infection of rabbit and guinea pig kidney tissue culture cells with HSV strains derived from brain or lip lesions at a high input multiplicity of virus failed to yield the "G" antigen. Infection of human kidney tissue culture cells with the same HSV strains yielded two units of the "G" antigen at 6 hr and 32 units at 24 hr. The conclusion that this was, nevertheless, not a new antigen, but only an increase in amount of preexisting antigen, was based on the demonstration that absorption of the anti-"HeLa G" globulin with uninfected human kidney tissue culture cells completely removed the antibody for the "G" antigen formed during the course of infection with HSV and also that present in uninfected WISH and HEp2 cells. Infection by the same HSV strains of the human WISH cells which have a small amount of preexisting "G" antigen resulted in an increase which was maximal at 24 hr. Infection of WISH cells with vaccinia virus resulted in a rapid increase of "G" antigen, which was demonstrable at 10 min but not at 3, 6, and 24 hr. Absorption of the anti-"HeLa G" globulin with uninfected WISH cells removed the antibody for the G antigen present in the WISH cells before infection as well as after infection with the herpes and vaccinia viruses, and also the antigen present in human tissue culture cells of malignant origin.

摘要

在旨在确定某些人疱疹病毒（HSV）毒株感染正常细胞是否会产生一种特定的新抗原（推测为人类恶性细胞所特有，即“HeLa G”）的实验过程中，发现这种新抗原的表面出现实际上仅代表先前存在的正常抗原组浓度的增加。针对该抗原的补体结合试验是用麦肯纳制备的抗体进行的，该抗体针对用氟碳通用电子（fluorocarbon genetron）纯化的HeLa细胞组分。这种抗体与源自各种人类恶性肿瘤的组织培养细胞提取物以及源自正常人类羊膜的自发转化WISH细胞系发生反应，但不与源自正常人肾早期传代培养物的细胞、幼兔肾原代培养物或新生豚鼠肾原代培养物的细胞发生反应。以高病毒输入复数用源自脑或唇部病变的HSV毒株感染兔和豚鼠肾组织培养细胞，未能产生“G”抗原。用相同的HSV毒株感染人肾组织培养细胞，在6小时时产生两个单位的“G”抗原，在24小时时产生32个单位。然而，得出这不是一种新抗原，而只是先前存在抗原量增加的结论，是基于以下证明：用未感染的人肾组织培养细胞吸收抗“HeLa G”球蛋白，可完全去除在HSV感染过程中形成的针对“G”抗原的抗体，以及未感染的WISH和HEp2细胞中存在的该抗体。用相同的HSV毒株感染预先存在少量“G”抗原的人WISH细胞，导致其增加，在24小时时达到最大值。用痘苗病毒感染WISH细胞导致“G”抗原迅速增加，在10分钟时可检测到，但在3、6和24小时时未检测到。用未感染的WISH细胞吸收抗“HeLa G”球蛋白，可去除感染前和感染疱疹病毒及痘苗病毒后WISH细胞中存在的针对G抗原的抗体，以及恶性来源的人组织培养细胞中存在的抗原。