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1
Improvements in the growth of BHK-21 cells in submerged culture.悬浮培养中BHK - 21细胞生长的改善。
Appl Microbiol. 1971 Oct;22(4):534-7. doi: 10.1128/am.22.4.534-537.1971.
2
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3
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Growth of the IB-RS-2 pig kidney cell line in suspension culture and its susceptibility to foot-and-mouth disease virus.IB-RS-2猪肾细胞系在悬浮培养中的生长及其对口蹄疫病毒的敏感性。
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5
Stoichiometry, kinetics, and regulation of glucose and amino acid metabolism of a recombinant BHK cell line in batch and continuous cultures.分批培养和连续培养中重组BHK细胞系葡萄糖和氨基酸代谢的化学计量学、动力学及调控
Biotechnol Prog. 1997 Jul-Aug;13(4):453-63. doi: 10.1021/bp970032z.
6
The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium.BHK细胞对基于非产氨型谷氨酸的培养基的适应性。
Biotechnol Bioeng. 1999 Aug 5;64(3):298-309.
7
Chemically characterized media for study of foot-and-mouth disease virus in baby hamster kidney cells.用于在幼仓鼠肾细胞中研究口蹄疫病毒的化学特性明确的培养基。
Appl Microbiol. 1967 Mar;15(2):228-32. doi: 10.1128/am.15.2.228-232.1967.
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Human 293 cell metabolism in low glutamine-supplied culture: interpretation of metabolic changes through metabolic flux analysis.低谷氨酰胺供应培养条件下的人293细胞代谢：通过代谢通量分析解读代谢变化
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10
Effects of L-glutamine deprivation on growth of HVJ (Sendai virus) in BHK cells.L-谷氨酰胺缺乏对仙台病毒（HVJ）在BHK细胞中生长的影响。
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引用本文的文献

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Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture.悬浮培养BHK-21细胞高效接种体制备阶段的方法
Cytotechnology. 2016 Jan;68(1):95-104. doi: 10.1007/s10616-014-9756-6. Epub 2014 Jun 19.
2
Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism.搅拌罐生物反应器中的通气-均化系统、溶解氧浓度和 pH 控制方式对 BHK-21 细胞生长和代谢的影响。
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3
The effect of Pluronic F-68 on hybridoma cells in continuous culture.普朗尼克F-68对连续培养的杂交瘤细胞的影响。
Appl Microbiol Biotechnol. 1992 Apr;37(1):44-5. doi: 10.1007/BF00174201.
4
Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.将BHK - 21细胞适应摇瓶培养生长并随后用日本脑炎病毒进行攻击。
Appl Microbiol. 1975 Sep;30(3):433-8. doi: 10.1128/am.30.3.433-438.1975.
5
Pluronic polyols in human lymphocyte cell line cultures.人淋巴细胞系培养中的普朗尼克多元醇
J Clin Microbiol. 1975 Jul;2(1):11-3. doi: 10.1128/jcm.2.1.11-13.1975.

本文引用的文献

1
Determination of glucose by an improved enzymatic procedure.采用改进的酶法测定葡萄糖。
Clin Chem. 1961 Oct;7:542-5.
2
Growth of L cell suspensions on a Warburg apparatus.L细胞悬液在瓦氏呼吸仪上的生长情况。
Proc Soc Exp Biol Med. 1963 Apr;112:1027-30. doi: 10.3181/00379727-112-28243.
3
Mechanism of D-glutamyltransferase repression in mammalian cells.
Biochim Biophys Acta. 1963 Feb 12;67:334-6. doi: 10.1016/0006-3002(63)91836-5.
4
An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels.一种用于测定血液、脑脊液和尿液葡萄糖水平的改良葡萄糖氧化酶法。
Clin Chim Acta. 1959 May;4(3):395-400. doi: 10.1016/0009-8981(59)90110-x.
5
The inhibition by glutamine of glutamyl transferase formation in cultures of human cells.
Biochim Biophys Acta. 1958 Feb;27(2):435-6. doi: 10.1016/0006-3002(58)90367-6.
6
The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate.分批培养和恒化器培养过程中小鼠细胞（LS株）对氨基酸的摄取：细胞生长速率的影响。
Proc R Soc Lond B Biol Sci. 1967 Nov 14;168(1013):421-38. doi: 10.1098/rspb.1967.0073.

悬浮培养中BHK - 21细胞生长的改善。

Improvements in the growth of BHK-21 cells in submerged culture.

作者信息

Radlett P J, Telling R C, Stone C J, Whiteside J P

出版信息

Appl Microbiol. 1971 Oct;22(4):534-7. doi: 10.1128/am.22.4.534-537.1971.

DOI:10.1128/am.22.4.534-537.1971
PMID:4331766
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376357/
Abstract

A medium is described which will support the submerged culture of BHK-21 cells to 6.5 x 10(6) to 7 x 10(6) cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 x 10(6) to 7 x 10(6) cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.

摘要

描述了一种培养基，它能支持BHK - 21细胞的深层培养，细胞密度可达6.5×10⁶至7×10⁶个细胞/毫升，培养体积可达30升。使用摇瓶培养来确定耗尽培养基中的营养缺陷。最终培养基在细胞密度为6.5×10⁶至7×10⁶个细胞/毫升时受到谷氨酰胺的限制，但增加谷氨酰胺浓度并不能提高细胞产量。说明了多元醇普朗尼克F68作为一种保护物质的作用。