悬浮培养中BHK - 21细胞生长的改善。
Improvements in the growth of BHK-21 cells in submerged culture.
作者信息
Radlett P J, Telling R C, Stone C J, Whiteside J P
出版信息
Appl Microbiol. 1971 Oct;22(4):534-7. doi: 10.1128/am.22.4.534-537.1971.
Abstract
A medium is described which will support the submerged culture of BHK-21 cells to 6.5 x 10(6) to 7 x 10(6) cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 x 10(6) to 7 x 10(6) cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.
摘要
描述了一种培养基，它能支持BHK - 21细胞的深层培养，细胞密度可达6.5×10⁶至7×10⁶个细胞/毫升，培养体积可达30升。使用摇瓶培养来确定耗尽培养基中的营养缺陷。最终培养基在细胞密度为6.5×10⁶至7×10⁶个细胞/毫升时受到谷氨酰胺的限制，但增加谷氨酰胺浓度并不能提高细胞产量。说明了多元醇普朗尼克F68作为一种保护物质的作用。
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