al-Rubeai M, Emery A N, Chalder S
Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK.
Appl Microbiol Biotechnol. 1992 Apr;37(1):44-5. doi: 10.1007/BF00174201.
The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.
研究了普朗尼克对杂交瘤细胞生长的比较作用。与未添加普朗尼克的培养物相比，添加0.05%的普朗尼克使连续培养中的稳态细胞数量减少了约12%。在短期实验中，结果表明随着普朗尼克浓度的增加，细胞数量逐渐减少。发现这种生长抑制是DNA合成速率降低的结果。
