溶液中吡啶核苷酸的结构。
A structure of pyridine nucleotides in solution.
作者信息
Oppenheimer N J, Arnold L J, Kaplan N O
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3200-5. doi: 10.1073/pnas.68.12.3200.
Abstract
Re-examination of the structure of pyridine coenzymes in solution by use of the 220-MHz high-frequency nuclear magnetic resonance spectrometer indicates that there is primarily one folded structure that is in rapid equilibrium with an open form. Reduced DPN(+) and reduced analogs of DPN(+) exist predominantly with the B side of the dihydropyridine ring folded against the adenine moiety. (The oxidized coenzymes appear to exist in the same folded structure.) Furthermore, the ribose protons undergo very little conformational change upon reduction of the pyridine ring; this observation strongly suggests a considerable similarity between the folded forms of the oxidized and reduced coenzymes. A model of the folded structure is presented.
摘要
利用220兆赫高频核磁共振光谱仪对溶液中吡啶辅酶的结构进行重新研究表明，主要存在一种折叠结构，它与开放形式处于快速平衡状态。还原型二磷酸吡啶核苷酸（DPN(+)）及其还原类似物主要以二氢吡啶环的B面折叠靠向腺嘌呤部分的形式存在。（氧化型辅酶似乎也以相同的折叠结构存在。）此外，吡啶环还原时核糖质子的构象变化很小；这一观察结果有力地表明氧化型和还原型辅酶的折叠形式之间存在相当大的相似性。本文提出了一种折叠结构模型。
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