Clifford W J, Anellis A
Appl Microbiol. 1971 Nov;22(5):856-61. doi: 10.1128/am.22.5.856-861.1971.
A biphasic culture medium suitable for cultivation and sporulation of Clostridium perfringens, C. botulinum, and C. sporogenes was devised. The medium designed for use in a disposable, compartmented, plastic film container contained peptones, yeast extract, minerals, an anion exchange resin, and glucose in 4% agar as the solid phase and (NH(4))(2)SO(4) and 0.1% agar as the liquid phase. With the biphasic system, it was not necessary to use active cultures as inocula. Growth was at least equal to that obtained in conventional media, and spore production of 9 out of 12 strains of C. perfringens equalled or usually exceeded that of conventional media.
设计出了一种适用于产气荚膜梭菌、肉毒梭菌和生孢梭菌培养及产孢的双相培养基。该培养基设计用于一次性的、分隔的塑料薄膜容器中，含有蛋白胨、酵母提取物、矿物质、阴离子交换树脂以及4%琼脂中的葡萄糖作为固相，硫酸铵和0.1%琼脂作为液相。使用双相系统时，无需使用活性培养物作为接种物。生长情况至少与传统培养基中获得的生长情况相当，并且12株产气荚膜梭菌中有9株的孢子产量等于或通常超过传统培养基中的产量。
