Clifford W J, Anellis A, Ross E W
Appl Microbiol. 1974 Apr;27(4):784-92. doi: 10.1128/am.27.4.784-792.1974.
Two basal media, containing the ingredients found in common in both SPS (BBL) and TSN (BBL) media and in the previously described media of Schaedler et al. (1965) and Starr et al (1971), but minus antibiotics, were selected as the most suitable for the enumeration of Clostridium perfringens spores in a model system. These media were also used to study the influence of the presence of glucose, xylose, or ribose in various concentrations (0, 0.01, 0.1, and 1.0%) on colony morphology and spore recovery. As the sugar concentration in the basal agar medium increased, the colonies of all the test organisms also increased in size, and more of the black colonies became white in color. At the 1.0% sugar level, glucose permitted only white colony development, whereas the pentoses were completely inhibitory. Both pour plates and most-probable-number tubes were inoculated with the spores of several strains of C. perfringens and incubated at 20, 30, 37, and 45 C for 24, 48, and 72 h. Statistical analyses of the enumeration data indicated, at the 99% confidence level, that a Trypticase(BBL)-yeast extract-glucose-sulfite-iron agar gave maximal population estimates at 37 C in 72 h.
选择了两种基础培养基，它们含有SPS（BBL）和TSN（BBL）培养基以及Schaedler等人（1965年）和Starr等人（1971年）之前描述的培养基中常见的成分，但不含抗生素，被认为最适合在模型系统中对产气荚膜梭菌孢子进行计数。这些培养基还用于研究不同浓度（0、0.01、0.1和1.0%）的葡萄糖、木糖或核糖的存在对菌落形态和孢子回收率的影响。随着基础琼脂培养基中糖浓度的增加，所有测试微生物的菌落大小也增加，并且更多的黑色菌落变成白色。在1.0%的糖水平下，葡萄糖仅允许白色菌落生长，而戊糖则完全具有抑制作用。将几种产气荚膜梭菌菌株的孢子接种到倾注平板和最大可能数管中，并在20、30、37和45℃下孵育24、48和72小时。对计数数据的统计分析表明，在99%的置信水平下，胰蛋白酶（BBL）-酵母提取物-葡萄糖-亚硫酸盐-铁琼脂在37℃下72小时可给出最大的种群估计数。
