用于产气荚膜梭菌计数的改良培养基。
Improved medium for enumeration of Clostridium perfringens.
作者信息
Harmon S M, Kautter D A, Peeler J T
出版信息
Appl Microbiol. 1971 Oct;22(4):688-92. doi: 10.1128/am.22.4.688-692.1971.
An improved selective medium, Tryptose-sulfite-cycloserine (TSC) agar, for the enumeration of Clostridium perfringens is described. It consists of the same basal medium as Shahidi-Ferguson-perfringens (SFP) agar, but with 400 mug of D-cycloserine per ml substituted for the kanamycin and polymyxin. Tolerance of C. perfringens for D-cycloserine, its production of lecithinase, and its ability to reduce sulfite were used as the basis for development of this medium. Comparisons were made between TSC and SFP agars for the recovery of vegetative cells of C. perfringens by using statistical methods. The results showed that TSC allowed virtually complete recovery of most of the C. perfringens strains while inhibiting practically all facultative anaerobes tested. SFP agar allowed a slightly higher rate of recovery of C. perfringens but was found to be much less selective.
本文描述了一种改良的选择性培养基——胰蛋白胨-亚硫酸盐-环丝氨酸（TSC）琼脂，用于产气荚膜梭菌的计数。它由与沙希迪-弗格森-产气荚膜梭菌（SFP）琼脂相同的基础培养基组成，但每毫升用400微克D-环丝氨酸替代了卡那霉素和多粘菌素。产气荚膜梭菌对D-环丝氨酸的耐受性、其卵磷脂酶的产生以及还原亚硫酸盐的能力被用作该培养基开发的基础。通过统计方法比较了TSC琼脂和SFP琼脂对产气荚膜梭菌营养细胞的回收率。结果表明，TSC能使大多数产气荚膜梭菌菌株几乎完全回收，同时几乎抑制了所有测试的兼性厌氧菌。SFP琼脂对产气荚膜梭菌的回收率略高，但选择性要低得多。
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