Adler J L, Finland M
Appl Microbiol. 1971 Nov;22(5):870-5. doi: 10.1128/am.22.5.870-875.1971.
The susceptibilities of recently isolated strains of Pseudomonas aeruginosa to gentamicin, polymyxin B, carbenicillin, ampicillin, penicillin G, and two newer penicillins were tested with the inocula-replicating technique by using undiluted and 10(-3) dilutions of the cultures. With either inoculum, polymyxin B was the most active agent, and a comparison with previous data from this laboratory showed that the susceptibility of P. aeruginosa to this antibiotic had not changed over the past 20 years. Gentamicin was nearly as active as polymyxin, all but 2 of the 141 strains tested with the diluted inoculum being inhibited by 6.25 mug/ml or less. AB-2288, an agent resembling carbenicillin, was four times more active than carbenicillin or BLP-1654; the last two were equally active against the 10(-3) inoculum. A more marked inoculum effect was noted with the penicillin analogues tested, the increase in minimum inhibiting concentration with the undiluted culture being eight-fold for carbenicillin and at least 16-fold for AB-2288 and BLP-1654. Pyocin typing and serotyping failed to demonstrate any clearly predominating types.
采用接种物复制技术，用未稀释的培养物和10⁻³稀释度的培养物测试了最近分离的铜绿假单胞菌菌株对庆大霉素、多粘菌素B、羧苄青霉素、氨苄青霉素、青霉素G和两种新型青霉素的敏感性。无论使用哪种接种物，多粘菌素B都是最有效的药物，与本实验室以前的数据比较表明，在过去20年中铜绿假单胞菌对这种抗生素的敏感性没有变化。庆大霉素的活性几乎与多粘菌素相同，用稀释接种物测试的141株菌株中，除2株外，其余均被6.25μg/ml或更低浓度抑制。AB - 2288，一种类似羧苄青霉素的药物，活性比羧苄青霉素或BLP - 1654高4倍；后两者对10⁻³接种物的活性相同。在所测试的青霉素类似物中观察到更明显的接种物效应，未稀释培养物的最低抑菌浓度增加，羧苄青霉素为8倍，AB - 2288和BLP - 1654至少为16倍。细菌素分型和血清分型未能显示出任何明显占优势的类型。
