Maruyama H B, Hatanaka M, Gilden R V
Proc Natl Acad Sci U S A. 1971 Sep;68(9):1999-2001. doi: 10.1073/pnas.68.9.1999.
Tumor virus RNAs from several mammalian and one reptilian species were purified; their 3'-terminal nucleoside was identified by separation of the trialcohols produced by periodate oxidation followed by reduction and tritiation with NaB(3)H(4). Each virus contained uridine as the predominant terminal nucleoside. Molecular weight estimations based on the tritiation reactions were consistent with a structure consisting of four subunits.
从几种哺乳动物和一种爬行动物中纯化出肿瘤病毒RNA;通过高碘酸盐氧化产生的三醇经还原并用NaB(3)H(4)进行氚化后进行分离,从而鉴定其3'-末端核苷。每种病毒都以尿苷作为主要的末端核苷。基于氚化反应的分子量估计与由四个亚基组成的结构一致。