Overby L R, Barlow G H, Doi R H, Jacob M, Spiegelman S
J Bacteriol. 1966 Sep;92(3):739-45. doi: 10.1128/jb.92.3.739-745.1966.
Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. II. Properties of the nucleic acids and coat proteins. J. Bacteriol. 92:739-745. 1966.-The ribonucleic acid (RNA) molecules and coat proteins of two RNA coliphages, MS-2 and Qbeta, have been characterized. MS-2 RNA shows an S(20,w) of 25.8 and a molecular weight by light scattering of 10(6). The corresponding parameters for Qbeta-RNA were 28.9 and 0.9 x 10(6). A difference in base composition was reflected in the adenine-uracil ratio, which was 0.95 for MS-2 and 0.75 for Qbeta. The two RNA preparations are readily separated by chromatography on columns of methylated albumin. Both gave identical bouyant densities in cesium sulfate of 1.64 g/ml. The coat protein subunits were of similar molecular weights: 15,500 (Qbeta) and 14,000 (MS-2). They differed, however, in that the Qbeta-protein lacked tryptophan and histidine, whereas the MS-2 protein lacked only histidine.
奥弗比，L.R.（伊利诺伊大学厄巴纳分校），G.H.巴洛，R.H.多伊，莫妮克·雅各布，和S.斯皮格尔曼。两种血清学不同的核糖核酸噬菌体的比较。II.核酸和外壳蛋白的特性。《细菌学杂志》92:739 - 745。1966年。——已对两种RNA大肠杆菌噬菌体MS - 2和Qβ的核糖核酸（RNA）分子及外壳蛋白进行了特性鉴定。MS - 2 RNA的沉降系数S(20,w)为25.8，通过光散射测得的分子量为10⁶。Qβ - RNA的相应参数分别为28.9和0.9×10⁶。碱基组成的差异反映在腺嘌呤 - 尿嘧啶比率上，MS - 2为0.95，Qβ为0.75。这两种RNA制剂在甲基化白蛋白柱上很容易通过色谱法分离。它们在硫酸铯中的浮力密度均为1.64克/毫升，相同。外壳蛋白亚基的分子量相似：Qβ为15,500，MS - 2为14,000。然而，它们的区别在于Qβ蛋白缺乏色氨酸和组氨酸，而MS - 2蛋白仅缺乏组氨酸。
