在缺乏DNA聚合酶I的甲苯处理的大肠杆菌中紫外线刺激的DNA合成
Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.
作者信息
Masker W E, Hanawalt P C
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):129-33. doi: 10.1073/pnas.70.1.129.
Abstract
Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive for DNA replication and additionally deficient in DNA polymerase I. UV-stimulated nonconservative synthesis is also seen in polA dnaE mutants that are deficient in two of the three known DNA polymerases. The observed UV-stimulated repair-like synthesis requires the presence of the four deoxyribonucleoside triphosphates and ATP. This mode of synthesis appears to differ from the ATP-independent nonconservative DNA synthesis previously reported to occur in toluenized bacteria.
摘要
在通过暴露于甲苯而对核苷三磷酸具有通透性的大肠杆菌中，已观察到紫外线（UV）刺激的非保守性DNA合成。这种合成在经甲苯处理的细胞中被检测到，在这些细胞中，通过使用对DNA复制温度敏感且另外缺乏DNA聚合酶I的dnaB突变体，可降低与UV无关的DNA合成背景。在缺乏三种已知DNA聚合酶中的两种的polA dnaE突变体中也观察到UV刺激的非保守性合成。观察到的UV刺激的类似修复的合成需要四种脱氧核糖核苷三磷酸和ATP的存在。这种合成模式似乎不同于先前报道的在经甲苯处理的细菌中发生的不依赖ATP的非保守性DNA合成。
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