Vladyko G V, Tsilinskiĭ Ia Ia
Vopr Virusol. 1979 Mar-Apr(2):169-74.
Fifteen ts mutants of Venezuelan equine encephalomyelitis (VEE) virus have been produced and studied. Two of them were isolated from a virus population grown in the presence of 5-fluorouracyl and 13 from a virus suspension treated with nitrous acid. The efficiency of plating 40 degrees/35 degrees of various mutants varied from 2 X 10(-2) to 6 X 10(-6). Three ts mutants had RNA- phenotype (RNA synthesis at a nonpermissive temperature was 1.5-3.3% of that of the wild type), 11 ts mutants had RNA+ phenotype (RNA synthesis decreased insignificantly, 32-75%), and one ts mutant was intermediate (18%).
已制备并研究了15种委内瑞拉马脑炎（VEE）病毒的温度敏感（ts）突变体。其中两种是从在5-氟尿嘧啶存在下生长的病毒群体中分离出来的，13种是从用亚硝酸处理过的病毒悬液中分离出来的。各种突变体在40℃/35℃下的铺板效率从2×10⁻²到6×10⁻⁶不等。三个ts突变体具有RNA⁻表型（在非允许温度下的RNA合成是野生型的1.5 - 3.3%），11个ts突变体具有RNA⁺表型（RNA合成无显著下降，为32 - 75%），还有一个ts突变体处于中间状态（18%）。
