Rosenthal P N, Robinson H L, Robinson W S, Hanafusa T, Hanafusa H
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336-40. doi: 10.1073/pnas.68.10.2336.
The 70S RNA component of several avian tumor viruses was hybridized with DNA extracted from avian tumor virus-infected and uninfected chicken and Japanese quail cells. Tritium-labeled 70S RNAs from Rous sarcoma virus (RSV), Rous associated virus-1 (RAV-1), RAV-60, and Schmidt-Ruppin-RSV (SR-RSV) hybridize from 3 to 10 times more with DNA from uninfected chicken cells than with DNA from Escherichia coli, calfthymus, or baby hamster kidney cells. After infection of chicken cells with RSV(RAV-1), SR-RSV, or RAV-2, the amount of 70S avian tumor virus [(3)H]RNA hybridized increases by 1.6 times. The specificity of the hybridization reaction was shown by the specific competition of 70S SR-RSV [(3)H]RNA with 70S RNA from RSV(RAV-1), and not with RNA from Sendai virus or chicken cells. There was no difference in the hybridization of 70S RNA from RSV (RAV-1), RAV-1, or RAV-60 with DNA either from chicken cells that contain RAV-60 in a nonreplicating form or from chicken cells that do not appear to contain RAV-60. These results indicate that both types of uninfected chicken cells contain DNA that is complementary to RNA from several avian tumor viruses and that the amount of complementary DNA increases in such cells after infection with an avian tumor virus. The RNAs of genetically different avian tumor viruses appear to have indistinguishable base sequences by this technique.
几种禽肿瘤病毒的70S RNA组分与从感染和未感染禽肿瘤病毒的鸡及日本鹌鹑细胞中提取的DNA进行杂交。来自劳氏肉瘤病毒（RSV）、劳氏相关病毒-1（RAV-1）、RAV-60和施密特-鲁平-RSV（SR-RSV）的氚标记70S RNA与未感染鸡细胞的DNA杂交的程度比与大肠杆菌、小牛胸腺或幼仓鼠肾细胞的DNA杂交的程度高3至10倍。用RSV（RAV-1）、SR-RSV或RAV-2感染鸡细胞后，杂交的70S禽肿瘤病毒[（3）H]RNA的量增加了1.6倍。70S SR-RSV[（3）H]RNA与RSV（RAV-1）的70S RNA特异性竞争，而不与仙台病毒或鸡细胞的RNA竞争，这表明了杂交反应的特异性。来自RSV（RAV-1）、RAV-1或RAV-60的70S RNA与以非复制形式含有RAV-60的鸡细胞或似乎不含RAV-60的鸡细胞的DNA杂交没有差异。这些结果表明，两种未感染的鸡细胞都含有与几种禽肿瘤病毒的RNA互补的DNA，并且在用禽肿瘤病毒感染后，此类细胞中互补DNA的量会增加。通过这种技术，遗传上不同的禽肿瘤病毒的RNA似乎具有难以区分的碱基序列。
