分化胎儿肝细胞原代培养的研究。
Studies on primary cultures of differentiated fetal liver cells.
作者信息
Leffert H L, Paul D
出版信息
J Cell Biol. 1972 Mar;52(3):559-68. doi: 10.1083/jcb.52.3.559.
Abstract
A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.
摘要
本文描述了一种培养未分化或生长缓慢的分化胎鼠肝细胞的方法。该方法使用胶原酶作为消化剂，并使用缺乏精氨酸的选择性培养基，这种培养基可抑制非实质肝细胞的生长。有证据表明，存活的细胞：(a) 通过将鸟氨酸转化为精氨酸的能力来保留肝脏特异性尿素循环功能；(b) 在缺乏葡萄糖的培养基中合成DNA；(c) 合成并分泌白蛋白。这种原代细胞培养物对部分肝切除大鼠的血清有反应，可能是研究肝脏再生调节机制的合适检测系统。
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