Eckert H, Jerochin S, Winsel K
Z Erkr Atmungsorgane. 1979;152(1):37-41.
Electron microscopical, autoradiographical and histochemical investigations were carried out in experimental lipid pneumonia, 6 weeks after exposure to mineral oil. In the lungs developed granulomatous changes with fibroblasts, some macrophages and leucocytes. With the aid of electron microscopy an increased number of fibroblasts with predominantly extracellular localized collagen fibers were visible in the granulomatous foci. Autoradiograms with 3H-Thymidine show an six-fold increase of number of radioactive labelled nuclei of fibroblasts in the granulomatous areas. This two methods--electron microscopy and autoradiography--appear sufficient for early detection of changes in experimental lung fibrosis.
在接触矿物油6周后的实验性脂质性肺炎中进行了电子显微镜、放射自显影和组织化学研究。肺部出现了肉芽肿性改变，有纤维母细胞、一些巨噬细胞和白细胞。借助电子显微镜，在肉芽肿病灶中可见数量增加的纤维母细胞，其主要有细胞外定位的胶原纤维。用3H-胸腺嘧啶核苷制作的放射自显影片显示，肉芽肿区域中纤维母细胞的放射性标记核数量增加了六倍。电子显微镜和放射自显影这两种方法似乎足以早期检测实验性肺纤维化的变化。
