磷酸从三磷酸腺苷转移至脑微粒体中与蛋白质结合的丝氨酸残基的酶促反应。
Enzyme transfer of phosphate from adenosine triphosphate to protein-bound serine residues in cerebral microsomes.
作者信息
Rodnight R, Lavin B E
出版信息
Biochem J. 1966 Nov;101(2):495-501. doi: 10.1042/bj1010495.
Abstract
- Microsomes from guinea-pig brain grey matter were incubated with [(32)P]ATP at 3mm concentration and the phosphate bound to the acid-washed, lipid-free residue was determined. 2. The binding process was Mg(2+)-dependent and resulted in the transfer of about 1-2 mmumoles of phosphate/mg. of protein/min. Under the conditions used univalent cations (Na(+),K(+) and Li(+)) inhibited the binding. 3. An unspecified proportion of this bound phosphate could be recovered in protein-derived phosphorylserine. The yield of labelled phosphorylserine was also decreased by univalent cations. 4. The bound phosphate formed with 3mm-MgATP was stable; addition of Na(+) or K(+) ions to the already labelled preparation had no effect on the bound phosphate level. 5. Bound phosphate was also formed when a solubilized fraction of the microsomes was incubated with ATP; univalent cations also inhibited this process. 6. p-Chloromercuribenzoate reduced the binding by about 25%; the inhibition was restored by cysteine.
摘要
- 将豚鼠脑灰质的微粒体与浓度为3mmol的[³²P]ATP一起孵育，然后测定与酸洗过的无脂质残渣结合的磷酸盐。2. 结合过程依赖于Mg²⁺，导致每毫克蛋白质每分钟转移约1 - 2微摩尔的磷酸盐。在所使用的条件下，单价阳离子（Na⁺、K⁺和Li⁺）会抑制结合。3. 这种结合的磷酸盐中有未指明比例的可以在蛋白质衍生的磷酸丝氨酸中回收。单价阳离子也会降低标记的磷酸丝氨酸的产量。4. 与3mmol - MgATP形成的结合磷酸盐是稳定的；向已经标记好的制剂中添加Na⁺或K⁺离子对结合磷酸盐水平没有影响。5. 当微粒体的可溶部分与ATP一起孵育时也会形成结合磷酸盐；单价阳离子也会抑制这个过程。6. 对氯汞苯甲酸使结合减少约25%；半胱氨酸可恢复这种抑制作用。
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