产碱韦荣球菌对核糖的利用
Ribose utilization by Veillonella alcalescens.
作者信息
Kafkewitz D, Delwiche E A
出版信息
J Bacteriol. 1972 Mar;109(3):1144-8. doi: 10.1128/jb.109.3.1144-1148.1972.
The utilization of ribose by Veillonella alcalescens has been further investigated. Nonfermentation of ribose is not a result of a phosphorylation lesion since ribose-phosphorylating activity was measured in cell extracts. Resting cells accumulated ribose-5-phosphate and nucleotides when (14)C-ribose was provided; no other sugar phosphates were detectable. Resting cells that were shifted to growth conditions polymerized rather than degraded the accumulated ribose compounds. Cell extracts contained a fructose diphosphate phosphatase. Ribose-5-phosphate, glucose-6-phosphate, and fructose-6-phosphate were not hydrolyzed. It is postulated that the nonfermentation of ribose is not due to any metabolic lesions, but is a consequence of metabolic control at the fructose diphosphate level of glycolysis.
对产碱韦荣球菌利用核糖的情况进行了进一步研究。核糖不发酵并非磷酸化损伤的结果，因为在细胞提取物中检测到了核糖磷酸化活性。当提供¹⁴C-核糖时，静息细胞积累了5-磷酸核糖和核苷酸；未检测到其他糖磷酸酯。转移到生长条件下的静息细胞聚合而非降解积累的核糖化合物。细胞提取物含有果糖二磷酸磷酸酶。5-磷酸核糖、6-磷酸葡萄糖和6-磷酸果糖未被水解。据推测，核糖不发酵并非由于任何代谢损伤，而是糖酵解中果糖二磷酸水平代谢控制的结果。
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