Weber J, Yohn D S
J Virol. 1972 Feb;9(2):244-50. doi: 10.1128/JVI.9.2.244-250.1972.
The paired radioiodine-labeled antibody technique (PRILAT) was applied to the detection and quantitation of avian tumor virus group-specific (gs) antigens and antibody. The technique proved to be specific, repeatable, and appreciably more sensitive than the microcomplement-fixation test for avian leukosis (COFAL). The PRILAT facilitated direct measurement of comparative antigen content of several types of transformed, neoplastic, or virus-infected cells and the magnitude of nonspecific antibody binding by appropriate control cells. The versatility of the technique was illustrated by application to the detection and quantitation of gs antibody content of chicken, turkey, pigeon, and hamster sera. Antibodies were detected in COFAL-negative sera from hamsters bearing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus. Sera from chickens bearing similar tumors were not positive for gs antibodies, although sera from turkeys and chickens immunized with avian leukosis virus did contain gs antibodies.
采用双份放射性碘标记抗体技术（PRILAT）检测和定量禽肿瘤病毒群特异性（gs）抗原及抗体。结果证明，该技术具有特异性、可重复性，且比禽白血病微量补体结合试验（COFAL）明显更灵敏。PRILAT有助于直接测定几种类型的转化细胞、肿瘤细胞或病毒感染细胞的相对抗原含量，以及通过适当的对照细胞测定非特异性抗体结合的程度。通过应用该技术检测和定量鸡、火鸡、鸽子和仓鼠血清中的gs抗体含量，说明了该技术的通用性。在感染劳氏肉瘤病毒施密特-鲁平株诱导肿瘤的仓鼠的COFAL阴性血清中检测到了抗体。患有类似肿瘤的鸡的血清中gs抗体未呈阳性，尽管用禽白血病病毒免疫的火鸡和鸡的血清中确实含有gs抗体。
