正常及病毒转化的仓鼠细胞系中的糖脂合成
Glycolipid synthesis in normal and virus-transformed hamster cell lines.
作者信息
Sakiyama H, Gross S K, Robbins P W
出版信息
Proc Natl Acad Sci U S A. 1972 Apr;69(4):872-6. doi: 10.1073/pnas.69.4.872.
Abstract
Studies have been continued on the synthesis of glycolipids by the NIL 2 line of hamster cells. Several clones were isolated from this line. These clones vary in morphology, saturation density, and glycolipid composition. Contrary to expectation there was no correlation between saturation density and complexity of the glycolipid pattern. In fact, the clone with the highest saturation density was the only one to show the complete set of glycolipids found previously in NIL 2 cells. All untransformed NIL clones show an increase in the level of "higher" (more than two saccharides/ceramide) glycolipids as the cells approach confluence. In the line containing all three "higher" neutral glycolipids, all three increased as cells approached saturation density. In the line containing only hematoside, this compound increased at confluence. Cells transformed by hamster sarcoma virus or polyoma virus showed no density-dependent glycolipid synthesis.
摘要
对仓鼠细胞NIL 2系合成糖脂的研究仍在继续。从该系中分离出了几个克隆。这些克隆在形态、饱和密度和糖脂组成方面存在差异。与预期相反，饱和密度与糖脂模式的复杂性之间没有相关性。事实上，饱和密度最高的克隆是唯一显示出先前在NIL 2细胞中发现的全套糖脂的克隆。所有未转化的NIL克隆在细胞接近汇合时，“高级”（超过两个糖/神经酰胺）糖脂水平都会增加。在含有所有三种“高级”中性糖脂的细胞系中，随着细胞接近饱和密度，所有三种糖脂都会增加。在仅含有血型糖苷的细胞系中，这种化合物在汇合时增加。由仓鼠肉瘤病毒或多瘤病毒转化的细胞未显示出密度依赖性糖脂合成。
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