Svoboda D, Reddy J
Am J Pathol. 1972 Jun;67(3):541-54.
Male rats were given 0.25% CPIB (ethyl chlorophenoxyisobutyrate) for 4 weeks at which time their liver cells showed the typical increase in number of microbodies (peroxisomes). During the first week after withdrawal of CPIB and simultaneous injection of allylisopropylacetamide (AIA), an inhibitor of catalase synthesis, the microbody matrix, in peroxidase preparations, showed marked decrease in electron opacity. In liver cells of rats from which CPIB was withdrawn but which were not given AIA, there were interruption in the limiting membrane and almost total loss of microbody matrix within 3 days. Simultaneous with the loss of matrix content, the hepatic catalase activity decreased from an average 80 units/mg protein to less than 10 units/mg protein. In liver cells of Mastomys natalensis, a multimammate rodent, withdrawal of CPIB was associated with the frequent occurrence of bare microbody nucleoids situated free in the hyaloplasm. The ultrastructural observations and the associated decrease in catalase activity suggest that one means of disposal of microbodies is by rather rapid dissolution or leakage of their matrix enzymes into the surrounding hyaloplasm.
给雄性大鼠喂食0.25%的CPIB（乙基氯苯氧基异丁酸酯），持续4周，此时它们的肝细胞显示出微体（过氧化物酶体）数量典型增加。在停用CPIB并同时注射过氧化氢酶合成抑制剂烯丙基异丙基乙酰胺（AIA）后的第一周，过氧化物酶制剂中的微体基质在电子密度上显示出明显降低。在停用CPIB但未给予AIA的大鼠肝细胞中，3天内界膜出现中断，微体基质几乎完全丧失。随着基质含量的丧失，肝脏过氧化氢酶活性从平均80单位/毫克蛋白质降至低于10单位/毫克蛋白质。在多乳鼠（一种多乳头啮齿动物）的肝细胞中，停用CPIB与透明质中频繁出现游离的裸露微体核仁有关。超微结构观察以及相关的过氧化氢酶活性降低表明，微体的一种处置方式是其基质酶相当迅速地溶解或泄漏到周围的透明质中。
