关于微体的新观察。对CPIB处理的大鼠肝脏的细胞化学研究。
New observations on microbodies. A cytochemical study on CPIB-treated rat liver.
作者信息
Legg P G, Wood R L
出版信息
J Cell Biol. 1970 Apr;45(1):118-29. doi: 10.1083/jcb.45.1.118.
Abstract
The liver of male rats has been studied after CPIB stimulation by using the peroxidase reaction for localizing catalase in hepatic cells. CPIB administration leads to an increase in the number of microbodies, and it is suggested that one mechanism by which microbody proliferation occurs is a process of fragmentation or budding from preexisting microbodies. Reaction product was observed not only within the microbody matrix, but outside the limiting membrane of the microbody and in association with ribosomes of adjacent rough endoplasmic reticulum. This localization of reaction product is interpreted as evidence that catalase after synthesis on rough endoplasmic reticulum may accumulate near microbodies and may be transferred directly into these organelles without traversing the cisternae of the endoplasmic reticulum or Golgi apparatus.
摘要
通过使用过氧化物酶反应来定位肝细胞中的过氧化氢酶，对雄性大鼠肝脏在CPIB刺激后进行了研究。给予CPIB会导致微体数量增加，有人提出微体增殖发生的一种机制是由预先存在的微体进行分裂或出芽的过程。不仅在微体基质内观察到反应产物，而且在微体的限制膜外以及与相邻糙面内质网的核糖体相关处也观察到了反应产物。反应产物的这种定位被解释为证据，表明在糙面内质网上合成后的过氧化氢酶可能在微体附近积累，并且可能直接转移到这些细胞器中，而无需穿过内质网或高尔基体的潴泡。
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