Gere J B, Krauth G H, Trahern C A, Bigham D A
Am J Clin Pathol. 1979 Mar;71(3):309-18. doi: 10.1093/ajcp/71.3.309.
A double-antibody radioimmunoassay that specifically measures serum or urinary concentrations of human cardiac myosin light chains has been developed. The assay is both sensitive and specific for myosin light chain I. The antiserum used in the assay is capable of detecting 1--2 ng/ml of cardiac myosin light chain I and shows no cross-reaction to cardiac myosin, the heavy chains of myosin, actin, tropomyosin, or light chain II. Of 114 patients admitted to the Coronary Care Unit, 227 measurements obtained from 84 patients without myocardial infarction revealed that the serum light chain level of 2.0 +/- 0.18 ng/ml was not significantly different from that of a control group (2.1 +/- 0.17 ng/ml, n = 12). In contrast, 89 measurements from 30 patients with myocardial infarction evidence clinically, electrocardiographically, and by creatine phosphokinase determinations were found to have an average serum light chain I concentration of 10.9 +/- 1.39 ng/ml (P less than 0.001). Thus, the radioimmunoassay for human cardiac myosin light chain I provides a sensitive and specific marker for myocardial damage.
已开发出一种特异性测量人心脏肌球蛋白轻链血清或尿液浓度的双抗体放射免疫测定法。该测定法对肌球蛋白轻链I既敏感又特异。测定中使用的抗血清能够检测到1-2纳克/毫升的心脏肌球蛋白轻链I，并且对心脏肌球蛋白、肌球蛋白重链、肌动蛋白、原肌球蛋白或轻链II无交叉反应。在114名入住冠心病监护病房的患者中，对84名无心肌梗死患者进行了227次测量，结果显示血清轻链水平为2.0±0.18纳克/毫升，与对照组（2.1±0.17纳克/毫升，n = 12）无显著差异。相比之下，对30名经临床、心电图及肌酸磷酸激酶测定证实有心肌梗死的患者进行了89次测量，发现血清肌球蛋白轻链I平均浓度为10.9±1.39纳克/毫升（P<0.001）。因此，人心脏肌球蛋白轻链I的放射免疫测定法为心肌损伤提供了一种敏感且特异的标志物。
