Uji Y, Sugiuchi H, Okabe H
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
J Clin Lab Anal. 1991;5(4):242-6. doi: 10.1002/jcla.1860050404.
A monoclonal solid-phase enzyme immunoassay has been developed for the detection of human serum ventricular myosin light chain-1. Cross-reactivity of this with human skeletal muscle myosin was observed, but the enzyme immunoassay with the sera of patients with acute myocardial infarction gave similar results with radioimmunoassay. The human ventricular myosin light chain-1 levels in the healthy subjects were 0.2-6.6 ng/ml in males and 0.2-4.1 ng/ml in females. Within-run and between-run precision (CVs) of the assays was on the order of 2.3-4.7% and 4.3-8.7, respectively. Sensitivity of the assay was 1.0 ng/ml, and working range was 5-100 ng/ml. In all patients with define acute myocardial infarction, serum ventricular myosin light chain-1 levels increased three- to ten-fold the upper reference range within 6 hr after the onset of chest pains. Two types of subtrend were discovered: its levels remain elevated for 3-4 days and its levels increased and then decreased 1-2 days after the initial rise but became elevated again for the next 4-7 days after the onset of chest pain, which is in contrast to the case with all conventionally used biochemical cardiac markers.
已开发出一种单克隆固相酶免疫测定法用于检测人血清中的心室肌球蛋白轻链-1。观察到其与人骨骼肌肌球蛋白存在交叉反应，但对急性心肌梗死患者血清进行的酶免疫测定与放射免疫测定结果相似。健康男性受试者的人心室肌球蛋白轻链-1水平为0.2 - 6.6 ng/ml，女性为0.2 - 4.1 ng/ml。该测定法的批内和批间精密度（变异系数）分别约为2.3 - 4.7%和4.3 - 8.7%。该测定法的灵敏度为1.0 ng/ml，工作范围为5 - 100 ng/ml。在所有明确诊断为急性心肌梗死的患者中，胸痛发作后6小时内，血清心室肌球蛋白轻链-1水平比参考范围上限升高了3至10倍。发现了两种亚趋势：其水平在3 - 4天内持续升高；其水平在最初升高后1 - 2天上升然后下降，但在胸痛发作后的接下来4 - 7天再次升高，这与所有传统使用的生化心脏标志物的情况不同。
