Llinás R, Blinks J R, Nicholson C
Science. 1972 Jun 9;176(4039):1127-9. doi: 10.1126/science.176.4039.1127.
Microinjection of aequorin, a bioluminescent protein sensitive tocalcium, into the presynaptic terminal of the squid giant synapse demnonstrated an increase in intracellular calcium ion concentration during repetitive synaptic transmission. Although no light flashes synchronous with individual presynaptic : tion potentials were detected, the results are considered consistent with the hypothesis that entry of calcium into the presynaptic terminal triggers release of e synaptic transmitter substance.
将水母发光蛋白（一种对钙敏感的生物发光蛋白）微量注射到枪乌贼巨大突触的突触前末端，结果表明在重复突触传递过程中细胞内钙离子浓度会升高。尽管未检测到与单个突触前动作电位同步的闪光，但这些结果被认为与钙进入突触前末端会触发神经递质释放这一假说相符。
